Occludin (OCLN) (AA 396-507) antibody

Antigen: Occludin (OCLN)

Synonyms: Ocl, AI503564, OCLN, ocln, oclnb, tpmt, DKFZp469D234

Binding Site: AA 396-507

Clonality: Monoclonal (19)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968360

Additional Information

Alternative name: Occludin

Immunogen: Mouse Occludin

Cross-Reactivity: Dog (Canine), Human, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 19

Description: Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intercellular barriers between epithelial and endothelial cells. Among the barriers formed by the tight junctions is the critical blood-brain barrier. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1 and ZO-2. The human occludin protein is 522 amino acids with four transmembrane domains in the first half of the protein. Two glycine/tyrosine-rich extracellular loops are involved in cell-cell interactions. In the cytoplasm, occludin interacts with ZO-1 through its intracellular C-terminus. A possible function of ZO-1 may be to link the adherens components, like occludin, to the submembraneous actin cytoskeleton. Experimental breakdown of the tight junctions at the blood-brain barrier results in redistribution of vinculin and loss of ZO-1 and Occludin. Taken together, these data indicate that occludin and ZO-1 are critical elements in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 65 kDA.

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of occludin on rat brain lysate. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of anti-occludin antibody.

Publications

Product: Ando-Akatsuka, Saitou, Hirase et al.: "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 43-7, 1996 (PubMed).

Bolton, Anthony, Perry: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, 1998 (PubMed).

Fanning, Jameson, Jesaitis et al.: "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29745-53, 1998 (PubMed).