CD38 (AA 171-292) antibody

Antigen: CD38

Synonyms: T10

Binding Site: AA 171-292

Clonality: Monoclonal (22)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968373

Additional Information

Immunogen: Human CD38

Format: Liquid

Isotype: IgG1, kappa

Clone: 22

Description: Lymphocyte trafficking is essential for efficient immune responses in that it allows antigen sensitive lymphocytes to encounter antigen and be recruited to sites of infection. Trafficking involves the passage of lymphocytes across the HEV, a process that is mediated by selectins. CD38 is a single-chain type II transmembrane glycoprotein that is expressed by a variety of immature and activated lymphocytes. It mediates a selectin-like binding to endothelial cells, thus functioning as an adhesion molecule. Human and mouse forms of the protein are highly similar and homologous to the Aplysia californica ADP-ribosyl-cyclase. CD38 catalyzes the synthesis of cyclic ADP-ribose (cADPR), a putative endogenous regulator of calcium release. This is thought to be key to CD38-mediated signal transduction. A possible ligand for CD38 is the Ig superfamily protein CD31. In addition, activation of CD38 with a monoclonal antibody induces leukemic cell proliferation. Thus, CD38 is a cell surface protein that is important for lymphocyte migration and may be involved in the propagation of leukemic cells in AML (acute myelogenous leukemia).

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 46 kDa

Comments:

Related Products: ABIN967389, ABIN968584

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of CD38 on EB1 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of CD38.

Immunofluorescence staining of Human Endothelial cells.

anti-CD38 (AA 171-292) antibody (Image 3)

Publications

Product: Jackson, Bell: "Isolation of a cDNA encoding the human CD38 (T10) molecule, a cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 7, pp. 2811-5, 1990 (PubMed).

Deaglio, Morra, Mallone et al.: "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 395-402, 1998 (PubMed).

Konopleva, Estrov, Zhao et al.: "Ligation of cell surface CD38 protein with agonistic monoclonal antibody induces a cell growth signal in myeloid leukemia cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4702-8, 1998 (PubMed).