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Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody

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Antigen
Synonyms zgc:109895, mvti1, vti1-rp2, DDBDRAFT_0219803, DDBDRAFT_0231536, DDB_0219803, DDB_0231536, MMDS3, MVti1, VTI1RP2, Vti1-rp2, 1110014F16Rik, 1110018K19Rik, 4921537J05Rik, MVti1a, Vti1
Epitope
AA 114-217
(23), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(52), (25), (16), (2), (1), (1)
Host
Mouse
(49), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(49), (30), (6), (5), (4), (3), (2), (2), (1)
Pubmed 5 references available
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Quantity 50 μg
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Immunogen Mouse Vti1a aa.114-217
Clone 45-Vti1a
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Vti1a (VTI1A Antibody Abstract)
Background Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.
Molecular Weight 29 kDa
Pathways
Comment

Related Products: ABIN967389, ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (ABIN968427) Western blot analysis of Vti1a on rat brain lysate. Lane 1: 1:2500, lane 2: 1:5000, l...
Immunofluorescence (IF) image for anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (ABIN968427) Immunofluorescence staining of NIH-3T3 cells.
 image for anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (ABIN968427) anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (Image 3)
Western Blotting (WB) image for anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (ABIN968427) anti-Vesicle Transport through Interaction with T-SNAREs Homolog 1A (Yeast) (VTI1A) (AA 114-217) antibody (Image 4)
Product cited in: Chiu, Novikov, Mukherjee et al.: "A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 637-48, 2002 (PubMed).

Shorter, Beard, Seemann et al.: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." in: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, 2002 (PubMed).

Mallard, Tang, Galli et al.: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." in: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, 2002 (PubMed).

Xu, Wong, Tang et al.: "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21783-9, 1998 (PubMed).

Advani, Bae, Bock et al.: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, 1998 (PubMed).

Catalog No. ABIN968427
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