Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) (AA 176-289) antibody

Antigen: Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1)

Synonyms: GR, GCR, GRL, GCCR, Grl1, Grl-1, Gcr, Grl, GR-A, NR3C1, NO, gcr, grl, xGR, gccr, gr, MGC113038, MGC191720, zgc:113038, GCL, Nr3c1, nr3c1, DKFZp469I0125, LOC100302444

Binding Site: AA 176-289

Clonality: Monoclonal (41)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Catalog no.: ABIN968430

Additional Information

Alternative name: Glucocorticoid Receptor

Immunogen: Human Glucocorticoid Receptor alpha

Format: Liquid

Isotype: IgG1

Clone: 41

Description: Steroid hormone receptors are hormone activated transcriptional regulators that influence genes required for embryonic development and adult homeostasis. One member of the steroid hormone family is the glucocorticoid receptor. It contains AF1 and AF2 transactivation domains, a DNA binding domain, and ligand binding domain. Ligand bound glucocorticoid receptors dimerize at specific palindromic sequences called glucocorticoid response elements (GREs) in the cis-regulatory region of target genes. Both AF1 and AF2 may be important for initiation or regulation of transcription by interacting with components of the initiation complex or other intermediary factors. In addition to transactivation, glucocorticoid receptors may also regulate transcription through transrepression of target genes. Although mechanisms of transrepression are not completely understood, DNA binding alone, DNA binding plus interaction with other transcription factors, or protein-protein interaction without DNA binding are mechanisms that have been implicated. Thus, glucocorticoid receptors function in the regulation of specific genes that are essential for human development and homeostasis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 94 kDa

Comments:

Related Products: ABIN967389, ABIN968535

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Hormones, Receptors

Restrictions: For Research Use only

Images

Western blot analysis of glucocorticoid receptor on HeLa cell lysate (right). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-glucocorticoid receptor.

Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol and the anti- Glucocorticoid Receptor antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken using a 20x objective and merged using the BD AttoVison ™ software.This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols.

Publications

Product: Hollenberg, Weinberger, Ong et al.: "Primary structure and expression of a functional human glucocorticoid receptor cDNA." in: Nature, Vol. 318, Issue 6047, pp. 635-41, 1986 (PubMed).

Beato, Herrlich, Schütz: "Steroid hormone receptors: many actors in search of a plot." in: Cell, Vol. 83, Issue 6, pp. 851-7, 1996 (PubMed).

Reichardt, Kaestner, Tuckermann et al.: "DNA binding of the glucocorticoid receptor is not essential for survival." in: Cell, Vol. 93, Issue 4, pp. 531-41, 1998 (PubMed).