p230 Trans Golgi (AA 2063-2179) antibody

Details for Product No. ABIN968452, Supplier: Log in to see
AA 2063-2179
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB)
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Immunogen Human p230 trans Golgi aa. 2063-2179
Clone 15-p230 trans Golgi
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The Golgi apparatus is a very complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. p230 trans Golgi is a peripheral membrane protein associated with the cytosolic face of the trans Golgi network (TGN). Protein sequence analysis indicates that it is highly hydrophilic and contains two proline-rich regions, as well as a high frequency of heptad repeats which are characteristic of alpha-helices that form dimeric coiled-coiled structures. Additionally, p230 trans Golgi contains a ESLALEELEL sequence, a motif of the acidic granin proteins that are found in secretory granules of neuroendocrine cells. p230 trans Golgi cycles between the cytosol and the TGN via non-clathrin coated vesicles in a G protein-dependent manner. The localization of p230 trans Golgi to the TGN is dependent on aromatic residues in its C-terminal non-coiled coil domain. Thus, p230 trans-Golgi is aTGN-specific protein that is important for the biogenesis of distinct populations of non-clathrin coated vesicles and, thus, functions in vesicular transport from the TGN. This antibody is routinely tested by western blot analysis.
Molecular Weight 230 kDa

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-p230 Trans Golgi (AA 2063-2179) antibody (ABIN968452) Western blot analysis of p230 trans-Golgi on a HeLa lysate. Lane 1: 1:250, lane 2: 1:...
 image for anti-p230 Trans Golgi (AA 2063-2179) antibody (ABIN968452) Immunoflouresence staining of HeLa cells.
Product cited in: Kjer-Nielsen, Teasdale, van Vliet, Gleeson: "A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins." in: Current biology : CB, Vol. 9, Issue 7, pp. 385-8, 1999 (PubMed).

Kjer-Nielsen, van Vliet, Erlich, Toh, Gleeson: "The Golgi-targeting sequence of the peripheral membrane protein p230." in: Journal of cell science, Vol. 112 ( Pt 11), pp. 1645-54, 1999 (PubMed).

Erlich, Gleeson, Campbell, Dietzsch, Toh: "Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8328-37, 1996 (PubMed).