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p230 Trans Golgi (AA 2063-2179) antibody

Details for Product No. ABIN968452
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Antigen
Epitope
AA 2063-2179
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
Pubmed 3 references available
Quantity 50 µg
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Catalog No. ABIN968452
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Immunogen Human p230 trans Golgi
Clone Ghr5
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background The Golgi apparatus is a very complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. p230 trans Golgi is a peripheral membrane protein associated with the cytosolic face of the trans Golgi network (TGN). Protein sequence analysis indicates that it is highly hydrophilic and contains two proline-rich regions, as well as a high frequency of heptad repeats which are characteristic of alpha-helices that form dimeric coiled-coiled structures. Additionally, p230 trans Golgi contains a ESLALEELEL sequence, a motif of the acidic granin proteins that are found in secretory granules of neuroendocrine cells. p230 trans Golgi cycles between the cytosol and the TGN via non-clathrin coated vesicles in a G protein-dependent manner. The localization of p230 trans Golgi to the TGN is dependent on aromatic residues in its C-terminal non-coiled coil domain. Thus, p230 trans-Golgi is aTGN-specific protein that is important for the biogenesis of distinct populations of non-clathrin coated vesicles and, thus, functions in vesicular transport from the TGN. This antibody is routinely tested by western blot analysis.
Molecular Weight 230 kDa
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-p230 Trans Golgi (AA 2063-2179) antibody Western blot analysis of p230 trans-Golgi on a HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p230 trans-Golgi antibody.
anti-p230 Trans Golgi (AA 2063-2179) antibody (2) Immunoflouresence staining of HeLa cells.
Product cited in: Erlich, Gleeson, Campbell et al.: "Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8328-37, 1996 (PubMed).

Kjer-Nielsen, Teasdale, van Vliet et al.: "A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins." in: Current biology : CB, Vol. 9, Issue 7, pp. 385-8, 1999 (PubMed).

Kjer-Nielsen, van Vliet, Erlich et al.: "The Golgi-targeting sequence of the peripheral membrane protein p230." in: Journal of cell science, Vol. 112 ( Pt 11), pp. 1645-54, 1999 (PubMed).

Validation Images
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