Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2) (AA 562-574) antibody
|Synonyms||GLT1, Eaat2, GLT-1, MGLT1, AI159670, 1700091C19Rik, 2900019G14Rik, Glt, Glt-1, EAAT, dEAAT2, DmelCG3159, CG3159, Eaat, GB16377, EAAT2, fj34b12, zgc:65897, wu:fj34b12, SLC1A2, DKFZp459A0719|
Alternatives Western Blotting (WB)
|4 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
Electrogenic-coupled (Na+ and K+) glutamate transporters in nerve terminals and glial cells are essential for maintaining subtoxic levels of extracellular excitatory amino acid neurotransmitters (e.g. glutamate and aspartate). They are also important for modifying synaptic transmission through the cotransport of ions and neurotransmitters. Excitatory amino acid transporter 2 (EAAT2) has 8 alpha-helical transmembrane domains and two reentrant pore-loop-like structures whose conformation is altered during ion neurotransmitter transport. EAAT2 is primarily expressed in astrocytes in adult brain and spinal cord, however it is also found in axons during fetal development. Mice deficient in EAAT2 exhibit lethal epileptic seizures with destruction of hippocampal neurons and an increased susceptibility to cold-induced cortical injury. Such defects in glutamine transporters have also been implicated in stroke, brain trauma, Alzheimer's disease, amyotrophic lateral sclerosis, and Huntington's disease. Thus, glutamine transporters, such as EAAT2, are critical for prevention of neurotoxic brain injury and may regulate glutamatergic synaptic transmission. This antibody is routinely tested by western blot analysis.
Synonyms: Excitatory amino acid transporter 2, Glutamate transporter 1, GLT1
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||66 kDa|
Related Products: ABIN968545, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Research Area||Neurology, Amino Acids, Signaling, Metabolism|
|Restrictions||For Research Use only|
Kirschner, Copeland, Gilbert et al.: "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." in: Genomics, Vol. 24, Issue 2, pp. 218-24, 1995 (PubMed).
Tanaka, Watase, Manabe et al.: "Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1." in: Science (New York, N.Y.), Vol. 276, Issue 5319, pp. 1699-702, 1997 (PubMed).
Yamada, Watanabe, Shibata et al.: "Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 15, pp. 5706-13, 1998 (PubMed).
Grunewald, Kanner: "The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9684-9, 2000 (PubMed).
|Hosts||Rabbit (36), Mouse (11), Guinea Pig (1)|
|Reactivities||Human (37), Rat (Rattus) (28), Mouse (Murine) (20), Cow (Bovine) (18), Horse (Equine) (18), Chicken (2), Dog (Canine) (2), Zebrafish (2), Arabidopsis thaliana (1), Barley (Hordeum vulgare) (1)|
|Applications||Western Blotting (WB) (27), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Immunohistochemistry (IHC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (8), N-Term (3), Internal Region (1), Met10 (1)|