Doublecortin (DCX) (AA 211-317) antibody
|Synonyms||DCX, 18C15.5, DC, DBCN, LISX, SCLH, XLIS, Dbct|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Description||The cerebral cortex is composed of multiple layers of neurons of distinct types and functions, which perform in unison to orchestrate cognitive function. The formation of the cortex relies on complex signaling mechanisms, which mediate the migration of newly formed neurons from deep within the brain to the superficial regions. Defects in neuronal migration and disruption of the multi-layered cortex are apparent in conditions such as X-linked lissencephaly (XLIS) and subcortical laminar heterotopia (SCLH) or double cortex (DC) syndrome. Mutations in the doublecortin gene have been linked to these brain disorders. Doublecortin is highly expressed in developing brain, primarily in migrating neurons. It is significantly homologous with the N-terminal region of the product of the KIAA0369 gene, a protein that is also similar to the CaM kinase family in its C-terminal region. Doublecortin has four potential MAP kinase family phosphorylation sites and a putative site for Abl tyrosine phosphorylation. Thus, doublecortin is thought to be an integral component of tyrosine kinase signal transduction pathways that regulate neuronal migration and development of the cerebral cortex.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||41 kDa|
Related Products: ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of Doublecortin on a mouse neonate lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-mouse Doublecortin antibody.|
des Portes, Pinard, Billuart et al.: "A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome." in: Cell, Vol. 92, Issue 1, pp. 51-61, 1998 (PubMed).
Gleeson, Allen, Fox et al.: "Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein." in: Cell, Vol. 92, Issue 1, pp. 63-72, 1998 (PubMed).
Magavi, Leavitt, Macklis: "Induction of neurogenesis in the neocortex of adult mice." in: Nature, Vol. 405, Issue 6789, pp. 951-5, 2000 (PubMed).