Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1) (AA 180-384) antibody

Details for Product No. ABIN968696
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Antigen
Synonyms IB1, JIP-1, Jip1, Prkm8ip, Skip, mjip-2a, JIP1, Jip-1, Mapk8ip, PRKM8IP
Epitope
AA 180-384
(10), (6), (4), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(43), (33), (30), (13), (13), (13)
Host
Mouse
(46), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(38), (24), (11), (9), (8), (6), (2), (1), (1)
Pubmed 3 references available
Quantity 50 µg
Options
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Catalog No. ABIN968696
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Immunogen Mouse JIP-1
Clone CD8
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name JIP-1
Background The JNK group of MAPKs is activated by a variety of inflammatory cytokines and environmental stressors. Activated JNK phosphorylates many cellular proteins, such as components of the AP-1 transcription factor complex (c-Jun and ATF-2). JNK-interacting proteins (JIPs) bind JNK, MKK7, MLKs, p190RhoGEF, and the Ste20-related protein kinase HPK1. In mouse, alternative splicing of JIP produces multiple splice variants, JIP-1, JIP-1b, JIP-2a, JIP-2b, and JIP-3. The structure of full length JIP consists of two N-terminal acidic regions, a JNK binding domain (JBD), two proline rich regions (PR), and both an SH3 and a phosphotyrosine-binding domain in the C-terminal region. JIP-1 localizes to the tips of neurites in differentiated PC12 cells, and may interact with JNK, MKK7, and MLK to facilitate formation of the JNK activating complex. IB1 is the rat homologue of JIP-1b, a JIP-1 variant that includes a 47 amino acid insert in the C-terminal region. IB1 is found in the nucleus and cytoplasm, and may function as a transactivator of the GLUT2 gene. Thus, JIP-1 and its related isoforms may have multiple functions that involve specific protein-protein interactions.
Synonyms: JNK Interacting Protein
Molecular Weight 112 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1) (AA 180-384) antibody Western blot analysis of JIP-1 on a mouse cerebellum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- JIP-1 antibody.
Product cited in: Dickens, Rogers, Cavanagh et al.: "A cytoplasmic inhibitor of the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 277, Issue 5326, pp. 693-6, 1997 (PubMed).

Bonny, Nicod, Waeber: "IB1, a JIP-1-related nuclear protein present in insulin-secreting cells." in: The Journal of biological chemistry, Vol. 273, Issue 4, pp. 1843-6, 1998 (PubMed).

Meyer, Liu, Margolis: "Interaction of c-Jun amino-terminal kinase interacting protein-1 with p190 rhoGEF and its localization in differentiated neurons." in: The Journal of biological chemistry, Vol. 274, Issue 49, pp. 35113-8, 2000 (PubMed).

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