Beclin (AA 171-291) antibody

Details for Product No. ABIN968744, Supplier: Log in to see
AA 171-291
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB)
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Immunogen Human Beclin aa. 171-291
Clone 20-Beclin
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Autophagy is the bulk degradation of cellular proteins through the autophagosomic-lysosomal pathway. This process is important for normal cell growth, and may be disrupted in tumor cells. Beclin is a Bcl-2 binding protein that is homologous to the yeast autophagy gene, apg6/vps30. The structure of beclin includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. However, inhibition of CRM1-dependent nuclear export leads to beclin localization primarily in the nucleus. Mutation of the NES domain of beclin also prevents nuclear export, as well as suppresses beclin-mediated nutrient deprivation-induced autophagy. In addition, these beclin mutants can not inhibit in vitro clonigenicity and in vivo tumorigenicity of MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since overexpression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects. Thus, beclin may be an important component of complexes involved in autophagy. This antibody is routinely tested by western blot analysis.
Molecular Weight 61 kDa

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Beclin (AA 171-291) antibody (ABIN968744) Western blot analysis of Beclin on a Jurkat lysate. Lane 1: 1:500, lane 2: 1:1000, la...
Product cited in: Liang, Yu, Brown, Levine: "Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor function." in: Cancer research, Vol. 61, Issue 8, pp. 3443-9, 2001 (PubMed).

Liang, Jackson, Seaman, Brown, Kempkes, Hibshoosh, Levine: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Aita, Liang, Murty, Pincus, Yu, Cayanis, Kalachikov, Gilliam, Levine: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Kleeman, Jiang, Gordon, Goldman, Berry, Herman, Levine: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

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