Images for product: anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202) antibody
A431 (ATCC CRL-1555) cells were either left untreated (lane 1) or treated (lane 2) with 100 ng/ml EGF for 5 minutes at 37°C. The top panel was probed with ERK1/2 (ABIN967700) and the bottom was probed with ERK1/2 (pT202/pY204) (ABIN968835).
Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 well imaging plate at ~10,000 cells per well. After overnight incubation, cells were either mock treated (media alone, left) or exposed to PMA (right) for 30 minutes. After treatment cells were stained using the alcohol perm protocol and the anti-ERK1/2 (pT202/pY204) antibody. The second step reagent was Alexa Fluor® 488 anti-mouse IgG. The image was taken on a BD Pathway™ 855 Bioimager with a 20x objective. This antibody also stains HeLa (ATCC CCL-2) and U-2 OS (ATCC HTB-96) cells and worked with both the Triton™ X-100 and alcohol perm protocols.