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Phosphoserine antibody

Details for Product No. ABIN968871, Supplier: Login to see New
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(116), (4), (3), (1), (1)
(73), (47)
Clonality (Clone)
Monoclonal ()
(11), (10), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(101), (71), (64), (29), (20), (17), (13), (11), (5), (1), (1)
Pubmed 3 references available
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Quantity 50 μg
Shipping to United States ( )
Clone 19-pSer
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Target Type Chemical
Background Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.
Research Area Neurology, Metabolism, Amino Acids, Protein Modifications
Application Notes Western blot: Typically, 1:2500 is a useful dilution for use in Western Blot.

Related Products: ABIN968887, ABIN967389, ABIN968871

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Immunofluorescence (IF) image for anti-Phosphoserine antibody (ABIN968871) Immunofluorescence. Top row: Anti-Phosphoserine (ABIN968871). Bottom Row: Phosphoseri...
Product cited in: Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).

Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).

Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).

Catalog No. ABIN968871
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