Phosphoserine antibody

Antigen: Phosphoserine

Clonality: Monoclonal (19)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968871

Additional Information

Format: Liquid

Isotype: IgG1

Clone: 19

Description: Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Comments:

Related Products: ABIN968887, ABIN967389, ABIN968871

Application Details

Application Notes: Western blot: Typically, 1:2500 is a useful dilution for use in Western Blot.

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Neurology, Metabolism, Amino Acids, Protein Modifications

Restrictions: For Research Use only

Images

Immunofluorescence. Top row: Anti-Phosphoserine (ABIN968871). Bottom Row: Phosphoserine/threonine (ABIN968873). first panels: untreated A431 cells control, second panels: A431 & CalyculinA/ Okadaic Acid (2 hour treatment).

Publications

Product: Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).

Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).

Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).