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Phosphoserine/threonine antibody

Details for Product No. ABIN968873
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Antigen
Reactivity
Human, Rat (Rattus)
(12)
Host
Mouse
(12)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(10), (3)
Pubmed 3 references available
Quantity 50 µg
Options
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Catalog No. ABIN968873
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Clone 22A
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine.
Research Area Neurology, Metabolism, Amino Acids
Application Notes For western blotting, a dilution of 1:2500 may be a useful starting concentration for titrations.
Comment

Related Products: ABIN968533, ABIN968887, ABIN967389, ABIN968871

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Phosphoserine/threonine antibody Immunofluorescence staining of phosphoserine (clone 19, MN ABIN968871) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
anti-Phosphoserine/threonine antibody (2) Immunofluorescence staining of phosphoserine (clone 19, MN ABIN968871) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.
anti-Phosphoserine/threonine antibody (3) Immunofluorescence staining of phosphoserine/threonine (clone 22A, MN ABIN968873) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
Product cited in: Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).

Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).

Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).

Validation Images
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