Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody

Antigen: Bruton Agammaglobulinemia tyrosine Kinase (BTK)

Synonyms: BTK, atk, bpk, xla, imd1, agmx1, psctk1

Clonality: Monoclonal (7F12H4)

Host: Mouse

Reactivity: Human, Monkey

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN968992

Additional Information

Alternative name: BTK

Gene ID: 695

Immunogen: Purified recombinant fragment of BTK expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 7F12H4

Description: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Synonyms: BTK

Molecular Weight: 77kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Immunology, Innate Immunity, Tyrosine Kinases

Restrictions: For Research Use only

Publications

Product: Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Thomas, Sideras, Smith et al.: "Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes." in: Science (New York, N.Y.), Vol. 261, Issue 5119, pp. 355-8, 1993 (PubMed).

Yamada, Kawakami, Kimura et al.: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).