Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody

Details for Product No. ABIN968992
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Antigen
Synonyms BTK, atk, bpk, xla, imd1, agmx1, psctk1, AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA, AI528679, xid
Reactivity
Human, Monkey
(163), (75), (68), (36), (36), (24), (6), (1)
Host
Mouse
(127), (35)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
(126), (62), (45), (40), (35), (33), (17), (16), (2), (1)
Pubmed 3 references available
Quantity 100 μL
Options
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Catalog No. ABIN968992
363.00 $
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Immunogen Purified recombinant fragment of BTK expressed in E. coli.
Clone 7F12H4
Isotype IgG1
Purification Ascites
Alternative Name BTK
Background Synonyms:
BTK
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Molecular Weight 77 kDa
Gene ID 695
Research Area Immunology, Innate Immunity, Tyrosine Kinases
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody Figure 3: Immunofluorescence analysis of Jurkat cells using BTK mouse mAb.
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody (2) Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using BTK mouse mAb with DAB staining.
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody (3) Figure 1: Western blot analysis using BTK mouse mAb against K562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysate.
Product cited in: Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Thomas, Sideras, Smith et al.: "Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes." in: Science (New York, N.Y.), Vol. 261, Issue 5119, pp. 355-8, 1993 (PubMed).

Yamada, Kawakami, Kimura et al.: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).

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