Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibody

Details for Product No. ABIN969035
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Antigen
Synonyms CDC27, NV10210, ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, HNUC, NUC2, AI452358, BC023187
Reactivity
Human
(111), (52), (48), (43), (28), (27), (24), (4), (1)
Host
Mouse
(94), (19)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(84), (47), (30), (26), (22), (9), (7), (5), (5), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μL
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Catalog No. ABIN969035
363.00 $
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Immunogen Purified recombinant fragment of human CDC27 expressed in E. coli.
Clone 5C12
Isotype IgG1
Purification Ascites
Alternative Name CDC27
Background Synonyms:
APC3, HNUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E,
Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Molecular Weight 91 kDa
Gene ID 996
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell Cycle, Coagulation
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibody Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibody (2) Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27 (AA: 724-830) -hIgGFc transfected HEK293 cell.
Product cited in: Goto, Eddy: "Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 42128-38, 2004 (PubMed).

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