Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody

Details for Product No. ABIN969052
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Antigen
Synonyms ZFH, Mi-2a, Mi2-ALPHA, 2600010P09Rik, AF020312, Chd7, Prp7, Prp9-1, CG9594, CHD-3, CHD3, DmCHD3, Dmel\\CG9594, chd3, dCHD3, LOC568230, zfh, mi-2a, mi2-alpha
Reactivity
Human, Mouse (Murine)
(40), (25), (16), (12), (11), (1)
Host
Mouse
(25), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(27), (21), (13), (11), (10), (9), (8), (8), (2), (2), (1)
Pubmed 1 reference available
Catalog no. ABIN969052
Quantity 0.1 mg
Price
363.00 $   Plus shipping costs $45.00
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of human CHD3 expressed in E. coli.
Clone 2G4
Isotype IgG1
Purification Ascites
Alternative Name CHD3
Background Synonyms:
ZFH, Mi-2a, Mi2-α, CHD3
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Molecular Weight 226 kDa
Gene ID 1107
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody Figure 4: Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple) .
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody (2) Figure 3: Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye.
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
anti-Chromodomain Helicase DNA Binding Protein 3 (CHD3) antibody (4) Figure 1: Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Product cited in: Burd, Kinyamu, Miller et al.: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).

Hosts (25), (18)
Reactivities (40), (25), (16), (12), (11), (1)
Applications (27), (21), (13), (11), (10), (9), (8), (8), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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