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DEAD Box Protein 4 (DDX4) antibody

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Antigen
Synonyms VASA, AV206478, Mvh, RVLG, vasa, vlg1
Reactivity
Human
(87), (35), (24), (13), (2), (2), (2), (1)
Host
Mouse
(68), (18), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(65), (34), (21), (15), (11), (11), (10), (5), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 100 μL
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Immunogen Purified recombinant fragment of human DDX4 expressed in E. coli.
Clone 2F9H5
Isotype IgG1
Purification Ascites
Alternative Name DDX4 (DDX4 Antibody Abstract)
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Molecular Weight 76 kDa
Gene ID 54514
HGNC 54514
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-DEAD Box Protein 4 (DDX4) antibody (ABIN969084) Figure 1: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-DEAD Box Protein 4 (DDX4) antibody (ABIN969084) Figure 2: Immunofluorescence analysis of MSCs (left) and NTERA-2 (right) cells using ...
Immunohistochemistry (IHC) image for anti-DEAD Box Protein 4 (DDX4) antibody (ABIN969084) Figure 3: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and...
Western Blotting (WB) image for anti-DEAD Box Protein 4 (DDX4) antibody (ABIN969084) Figure 4: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected ...
Product cited in: Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Shibata, Tsunekawa, Okamoto-Ito et al.: "Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein." in: Molecular reproduction and development, Vol. 67, Issue 1, pp. 1-7, 2003 (PubMed).

Zeeman, Stoop, Boter et al.: "VASA is a specific marker for both normal and malignant human germ cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 2, pp. 159-66, 2002 (PubMed).

Castrillon, Quade, Wang et al.: "The human VASA gene is specifically expressed in the germ cell lineage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9585-90, 2000 (PubMed).

Catalog No. ABIN969084
$393.80
Plus shipping costs $45.00

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