You are viewing an incomplete version of our website. Please click to reload the website as full version.

Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody

Details for Product No. ABIN969221, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms ITGA5, Cd49e, Fnra, VLA5, CD49e, FNRA, VLA5A, Itga5_retired
(192), (158), (40), (4), (3), (3), (3), (1)
(117), (82), (81), (31), (4)
Clonality (Clone)
Monoclonal ()
(38), (25), (20), (6), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(201), (121), (73), (70), (45), (43), (21), (17), (17), (11), (6), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of human ITGA5 expressed in E. coli.
Clone 10F6
Isotype IgG2a
Purification Ascites
Alternative Name ITGA5 (ITGA5 Antibody Abstract)
Background The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
Molecular Weight 114 kDa
Gene ID 3678
HGNC 3678
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (ABIN969221) Figure 1: Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and ne...
Immunohistochemistry (IHC) image for anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (ABIN969221) Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues us...
Western Blotting (WB) image for anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (ABIN969221) Figure 3: Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfecte...
Product cited in: Mould, Askari, Barton et al.: "Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19800-5, 2002 (PubMed).

Zhao, Newman: "Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 65-73, 2001 (PubMed).

Wong, Wiedle, Ballestrem et al.: "PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3109-21, 2000 (PubMed).

Background publications Byzova, Plow: "Activation of alphaVbeta3 on vascular cells controls recognition of prothrombin." in: The Journal of cell biology, Vol. 143, Issue 7, pp. 2081-92, 1999 (PubMed).

Jones, Gockerman, Busby et al.: "Insulin-like growth factor binding protein 1 stimulates cell migration and binds to the alpha 5 beta 1 integrin by means of its Arg-Gly-Asp sequence." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 22, pp. 10553-7, 1993 (PubMed).

Catalog No. ABIN969221
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)