Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody

Antigen: Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5)

Synonyms: CD11C, SLEB6, Cr4, N418, Cd11c, AI449405, ITGAX, ITGA5, CD11c

Clonality: Monoclonal (10F6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969221

Additional Information

Alternative name: ITGA5

Gene ID: 3678

Immunogen: Purified recombinant fragment of human ITGA5 expressed in E. Coli.

Format: Liquid

Isotype: IgG2a

Clone: 10F6

Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
Synonyms: FNRA, CD49e, VLA5A, ITGA5

Molecular Weight: 114kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: Jones, Gockerman, Busby et al.: "Insulin-like growth factor binding protein 1 stimulates cell migration and binds to the alpha 5 beta 1 integrin by means of its Arg-Gly-Asp sequence." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 22, pp. 10553-7, 1993 (PubMed).

Byzova, Plow: "Activation of alphaVbeta3 on vascular cells controls recognition of prothrombin." in: The Journal of cell biology, Vol. 143, Issue 7, pp. 2081-92, 1999 (PubMed).

Wong, Wiedle, Ballestrem et al.: "PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3109-21, 2000 (PubMed).

Zhao, Newman: "Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 65-73, 2001 (PubMed).

Mould, Askari, Barton et al.: "Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domain." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19800-5, 2002 (PubMed).