Kelch-Like 1 (Drosophila) (KLHL1) antibody

Antigen: Kelch-Like 1 (Drosophila) (KLHL1)

Synonyms: MRP2, FLJ30047, KIAA1490

Clonality: Monoclonal (3A8)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN969241

Additional Information

Alternative name: KLHL1

Gene ID: 57626

Immunogen: Purified recombinant fragment of human KLHL1 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 3A8

Description: The mammalian Kelch like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin organizing proteins. Based on the presence of anti sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved in this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.Tissue specificity: Highly expressed in brain.
Synonyms: MRP2, FLJ30047, KIAA1490, KLHL1

Molecular Weight: 82kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Publications

Product: Nagase, Kikuno, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 2, pp. 143-50, 2000 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Barrios-Rodiles, Brown, Ozdamar et al.: "High-throughput mapping of a dynamic signaling network in mammalian cells." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1621-5, 2005 (PubMed).