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Keratin 15 (KRT15) antibody

Details for Product No. ABIN969250, Supplier: Login to see
Antigen
  • xlk2
  • CK15
  • K15
  • K1CO
  • AI528832
  • Krt1-15
  • Ka15
  • wu:fa91c06
  • wu:fa95d04
  • zgc:55334
Reactivity
Human
94
34
32
8
2
1
1
Host
Mouse
57
34
8
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
69
42
33
28
24
11
10
8
4
3
2
1
Supplier
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Immunogen Purified recombinant fragment of KRT15 expressed in E. coli.
Clone 6-00E-07
Isotype IgG2a
Purification Ascites
Alternative Name KRT15 (KRT15 Antibody Abstract)
Background The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
Molecular Weight 49 kDa
Gene ID 3866
HGNC 3866
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-Keratin 15 (KRT15) antibody (ABIN969250) Figure 1: Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and ...
Immunocytochemistry (ICC) image for anti-Keratin 15 (KRT15) antibody (ABIN969250) Figure 2: Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using ...
Immunohistochemistry (IHC) image for anti-Keratin 15 (KRT15) antibody (ABIN969250) Figure 3: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues usin...
Western Blotting (WB) image for anti-Keratin 15 (KRT15) antibody (ABIN969250) Figure 4: Western blot analysis using KRT15 mouse mAb against A431 cell lysate.
Product cited in: Radoja, Stojadinovic, Waseem et al.: "Thyroid hormones and gamma interferon specifically increase K15 keratin gene transcription." in: Molecular and cellular biology, Vol. 24, Issue 8, pp. 3168-79, 2004 (PubMed).

Werner, Munz: "Suppression of keratin 15 expression by transforming growth factor beta in vitro and by cutaneous injury in vivo." in: Experimental cell research, Vol. 254, Issue 1, pp. 80-90, 2000 (PubMed).

Background publications Waseem, Dogan, Tidman et al.: "Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 362-9, 1999 (PubMed).