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Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibody

Details for Product No. ABIN969459
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Antigen
Synonyms WNT10B, Xwnt-10b, Xwnt10b, wnt-12, Wnt12, SHFM6, WNT-12, Xwnt-10, wnt10, wnt10-A, Zwnt[d], wnt10br, wnt[d]
Reactivity
Human
(45), (24), (21), (13), (13), (13)
Host
Mouse
(40), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
(32), (19), (18), (15), (10), (9), (5), (3), (1)
Pubmed 1 reference available
Quantity 100 µL
Options
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Catalog No. ABIN969459
407.00 $
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Immunogen Purified recombinant fragment of human WNT10B expressed in E. coli.
Clone 5A7
Isotype IgG1
Purification Ascites
Alternative Name WNT10B
Background Synonyms:
SHFM6, WNT-12
WNT10B: wingless-type MMTV integration site family, member 10B. The WNT family consists of structurally related secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is ikely a molecular switch that governs adipogenesis. This protein is 96 % identical to the mouse Wnt10B protein at the AA level. The WNT10B gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
Molecular Weight 43 kDa
Gene ID 7480
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibody Figure 3: Confocal immunofluorescence analysis of PANC-1 cells using WNT10B mouse mAb (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibody (2) Figure 2: Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using WNT10B mouse mAb with DAB staining.
anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) antibody (3) Figure 1: Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1) .
Product cited in: Saitoh, Kirikoshi, Mine et al.: "Proto-oncogene WNT10B is up-regulated by tumor necrosis factor alpha in human gastric cancer cell line MKN45." in: International journal of oncology, Vol. 19, Issue 6, pp. 1187-92, 2001 (PubMed).

Validation Images
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