RAB25, Member RAS Oncogene Family (RAB25) antibody

Details for Product No. ABIN969489
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Antigen
Synonyms CATX-8, RAB11C, AV012978, MGC84182, RAB25, catx-8, MGC79587, rab25, si:dkeyp-84f11.7, zgc:101648
Reactivity
Human
(38), (20), (13), (13), (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(19), (16), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(29), (17), (13), (10), (7), (5), (5), (4), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN969489
Quantity 100 µL
Price
451.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of Rab25 expressed in E. coli.
Clone 3F12F3
Isotype IgG2a
Purification Purified
Alternative Name Rab25
Background Synonyms:
CATX-8, RAB25
Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric pariηl cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa
Gene ID 57111
Research Area Cancer
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (A), breast carcinoma (B), esophagus carcinoma (C), skin carcinoma (D) tissue, showing cytoplasmic localization using Rab25 mouse mAb with DAB staining.
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody (2) Figure 1: Western blot analysis using Rab25 mouse mAb against truncated Rab25 recombinant protein (1), human overy carcinoma (2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.
Product cited in: Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).

Wang, Kumar, Navarre et al.: "Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 29138-46, 2000 (PubMed).

Hosts (19), (16), (3)
Reactivities (38), (20), (13), (13), (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (29), (17), (13), (10), (7), (5), (5), (4), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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