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Ubiquitin B antibody (UBB)

Details for Product anti-UBB Antibody No. ABIN969573, Supplier: Log in to see
Antigen
  • AL033289
  • Rps27a
  • Uba52
  • UBB
  • Ubb2
  • UBC
  • Ubc
  • Ubiquitin
Alternatives
anti-Human Ubiquitin B antibody for ELISA
Reactivity
Human
32
15
12
1
Host
Mouse
30
6
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
32
19
9
9
8
5
2
1
Supplier
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Immunogen Purified recombinant fragment of human UBB expressed in E. coli.
Clone 3C12
Isotype IgG1
Purification Purified
Alternative Name UBB (UBB Antibody Abstract)
Background This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Molecular Weight 26 kDa
Gene ID 7314
HGNC 7314
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Ubiquitin B antibody (UBB) (ABIN969573) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Ubiquitin B antibody (UBB) (ABIN969573) Figure 2: Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and nega...
Western Blotting (WB) image for anti-Ubiquitin B antibody (UBB) (ABIN969573) Figure 3: Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) ...
Western Blotting (WB) image for anti-Ubiquitin B antibody (UBB) (ABIN969573) Figure 4: Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombina...
Product cited in: Choudhary, Kumar, Gnad, Nielsen, Rehman, Walther, Olsen, Mann: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009

Komander: "The emerging complexity of protein ubiquitination." in: Biochemical Society transactions, Vol. 37, Issue Pt 5, pp. 937-53, 2009