RBC Lysis/Fixation Solution (10X)

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Intracellular Flow Cytometry (ICFC)
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Characteristics RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H2 0 prior to use.
Components RBC Lysis/Fixation Solution (10X)
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Buffer This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water. The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage 4 °C
Storage Comment Store RBC lysis buffer between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for RBC Lysis/Fixation Solution (10X) (ABIN2669206) RBC Lysis/Fixation Solution (10X)
Flow Cytometry (FACS) image for RBC Lysis/Fixation Solution (10X) (ABIN2669206) RBC Lysis/Fixation Solution (10X) (Image 2)
Background publications Kusner, Ciesielski, Marx, Kaminski, Fenstermaker: "Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study." in: PLoS ONE, Vol. 9, Issue 7, pp. e102231, 2014 (PubMed).