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RDH10 antibody

RDH10 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7118460
  • Target See all RDH10 Antibodies
    RDH10 (Retinol Dehydrogenase 10 (All-Trans) (RDH10))
    Reactivity
    • 33
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 33
    Rabbit
    Clonality
    • 33
    Polyclonal
    Conjugate
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RDH10 antibody is un-conjugated
    Application
    • 18
    • 15
    • 14
    • 13
    • 13
    • 12
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Purification
    Immunogen affinity purified
    Purity
    ≥95 % as determined by SDS-PAGE
    Immunogen
    retinol dehydrogenase 10(all-trans)
    Isotype
    IgG
  • Application Notes
    WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200
    Restrictions
    For Research Use only
  • Validation #104469 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Palczewski Lab, Center For Translational Vision Research, UC Irvine
    No.
    #104469
    Date
    03/23/2023
    Antigen
    RDH10
    Lot Number
    20221223E
    Method validated
    Immunohistochemistry
    Positive Control

    Retina cryosection from B6 Albino (B6(Cg)-Tyrc-2J/J) animal

    Negative Control

    Retina cryosection from B6 Albino (B6(Cg)-Tyrc-2J/J) animal

    No primary antibody

    Notes

    Passed. Presence of specific signal in the RPE cell layer was considered as indication of specific immunoreactivity using the anti-RDH10 antibody ABIN7118460.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN7118460
    Secondary Antibody
    donkey anti-rabbit AF647-conjugated antibody (Abcam, 150075)
    Full Protocol
    • Collect eyes from mice and fix with paraformaldehyde 4% (Electron Microscopy Sciences, 15710) in 1x PBS for 30 min at RT.
    • Cryoprotection with sucrose series:
      • Wash in 10% sucrose in 1x PBS.
      • Immerse in 10% sucrose in 1x PBS for 30 min at RT.
      • Wash in 20% sucrose in 1x PBS.
      • Immerse in 20% sucrose in 1x PBS for 30 min RT.
      • Wash in 30% sucrose in 1x PBS.
      • 30% sucrose ON at 4°C.
    • Embed eyes in OCT compound (Tissue-Tek O.C.T. Compound, 4583).
    • Cut retinal sections at a thickness of 12 μm on a cryostat.
    • Air dry sections for 15 min at RT, store at -80°C until use.
    • Bring sections to RT and rehydrate in 1x PBS for 1 h.
    • Incubate sections in blocking buffer (1x PBS, 3% BSA (Sigma-Aldrich, A7030), 3% Donkey serum (Sigma-Aldrich, S30-100ML), 0.1% Triton X-100 (Sigma-Aldrich, X100-500ML)) for 1 h at RT.
    • Incubate sections with primary rabbit anti-RDH10 antibody (antibodies-online, ABIN7118460, lot 20221223E) diluted 1:50 in blocking buffer ON at RT. Include a no primary antibody negative controls. Additionally, counterstaing with primary mouse anti-CRALBP antibody (Thermo Fisher Scientific, MA1-813).
    • Rinse sections 3 times with 1x PBS, 0.1% Triton X100. Keep negative controls in a separate container.
    • Incubate sections with secondary AF647-conjugated donkey anti-rabbit antibody (Abcam, Ab150075) or AF488-conjugated donkey anti-mouse antibody (Thermo Fisher Scientific, A32766) diluted 1:500 in blocking buffer for 1 h at RT.
    • Rinse sections once with 1x PBS, 0.1% Triton X-100 for 5 min at RT.
    • Incubate sections in 1x DAPI (Thermo Fisher Scientific, 62248) in 1x PBS, 0.1% Triton X-100 for 15 min at RT.
    • Rinse sections 3x with 1x PBS, 0.1% Triton X-100 for 5 min at RT.
    • Mount sections in VECTASHIELD® HardSet™ Antifade Mounting Medium (Vector Laboratories, H-1400) mounting medium.
    • Acquire images with a fluorescence microscope and appropriate filter settings.For the validation purposes Keyence BZ-X800E fluorescence microscope was used with following filters: BZ-X DAPI for DAPI, BZ-X GFP for AF488, BZ-X Cy5 for AF647. Images were taken at 10x and 40x magnification.
    Experimental Notes
    • Experiment involved validation of the specificity of 4 antibodies against different Rdh proteins: Rdh5 (ABIN7254060), Rdh10 (ABIN7118460), Rdh11 (ABIN966957), and Rdh12 (ABIN7167836). All 4 proteins are important for eye function and highly expressed in neural retina and/or RPE. Validation is based on comparison of each staining with known pattern of expression in the mouse retina. For review highlighting each Rdh localization see PMID20801113.

    • To aid orientation in the cell layers anti-Cralbp counterstain was included in the staining (Thermo MA1-813). Cralbp (Rlbp1) is highly expressed in RPE and Müller glia cells in mouse retina.

  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
    Expiry Date
    12 months
  • Tworak, Kolesnikov, Hong, Choi, Luu, Palczewska, Dong, Lewandowski, Brooks, Campello, Swaroop, Kiser, Kefalov, Palczewski: "Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia." in: Cell reports, Vol. 42, Issue 8, pp. 112982, (2023) (PubMed).

  • Target
    RDH10 (Retinol Dehydrogenase 10 (All-Trans) (RDH10))
    Alternative Name
    RDH10 (RDH10 Products)
    Synonyms
    cb804 antibody, rdh10 antibody, SDR16C4 antibody, 3110069K09Rik antibody, 4921506A21Rik antibody, AI875664 antibody, AW549993 antibody, D1Ertd762e antibody, m366Asp antibody, retinol dehydrogenase 10b antibody, retinol dehydrogenase 10 antibody, retinol dehydrogenase 10 (all-trans) antibody, rdh10b antibody, MCYG_00691 antibody, RDH10 antibody, Rdh10 antibody
    Background
    Synonyms:SDR16C4 Background:Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol.
    Molecular Weight
    39 kDa
    Gene ID
    157506
    UniProt
    Q8IZV5
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