Bilirubin Assay Kit

Details for Product No. ABIN1000256
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Target Name (Antigen)
Detection Range 0.16 mg/dL.
Application
Biochemical Assay (BCA)
Pubmed 14 references available
Catalog no. ABIN1000256
Quantity 180 tests
Price
273.90 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 4 to 6 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Sample Type Serum
Characteristics Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Components Reagent A: 30 mL. Reagent B: 10 mL. Reagent C: 30 mL. Saline: 50 mL. Calibrator: 2 mL (equivalent to 5 mg/dL Bilirubin).
Material not included Pipeting devices and accessories, 96-well plates and plate reader.
Background Quantitative determination of bilirubin by colorimetric (530nm) method.
Procedure: 10 min.

Bilirubin is one of the degradation products of hemoglobin formed when red blood cells die. Bilirubin exists in the insoluble unconjugated form (also indirect bilirubin), or soluble glucuronide conjugated form bilirubin (also direct bilirubin). Conjugated bilirubin moves into the bile canaliculi of the liver and then to the gall bladder. When stimulated by eating, bile (including the conjugated bilirubin) is excreted into the small intestine, where bilirubin is converted into urobilinogen. Bilirubin is a key diagnostic indicator. High levels of bilirubin result when too much hemoglobin is broken down or the removal of bilirubin does not function properly. The accumulation of bilirubin in the body causes jaundice. Simple and automation-ready procedures for quantitative determination of bilirubin find wide applications in research and drug discovery. This bilirubin assay kit is designed to measure bilirubin in blood specimen in 96-well or cuvette formats. The improved Jendrassik- Grof method utilizes the reaction of bilirubin with diazotized sulfanilic acid, in which a red colored product is formed. The intensity of the color, measured at 510-550nm, is an accurate measure of the bilirubin level in the sample. Total bilirubin is assessed using caffeine benzoate to split bilirubin from the unconjugated bilirubin protein complex.
Application Notes Direct Assays: total and direct bilirubin in serum or plasma.
Pharmacology: effects of drugs on bilirubin metabolism.
Protocol Procedure using 96-well plate:
1. Reagent Preparation: prepare at least 200 L/well. Total Bilirubin is determined with Working Reagent that contains Reagent C, and Direct Bilirubin with Working Reagent that does not contain Reagent C but saline instead.
2. Calibrator: transfer 50 L H2O and 50 L Calibrator into two wells of clear-bottom 96-well plate, add 200 L H2O. The volume in each well 250 L. Samples: transfer 50 L sample into separate wells, add 200 L respective Working Reagent (i.e. for total bilirubin and/or direct bilirubin) and 200 L Blank Reagent to the sample wells.
3. Incubate 10 min and read OD530nm (510 to 550nm).

Procedure using Cuvet:
1. Prepare at least 800 L/well fresh Working Reagent.
2. Transfer 200 L H2O and 200 L Calibrator into two cuvets, add 800 L H2O. Transfer 200 L sample into cuvet, add 800 L Working Reagent.
3. Incubate 10 min and read OD530nm (510 to 550nm).
Restrictions For Research Use only
Storage 4 °C
Product cited in: Sirica, Zhang, Lai et al.: "A novel \" model of cholangiocarcinoma progression based on bile duct inoculation of tumorigenic rat cholangiocyte cell lines." in: Hepatology (Baltimore, Md.), Vol. 47, Issue 4, pp. 1178-90, 2008 (PubMed).

Vinchi, Gastaldi, Silengo et al.: "Hemopexin prevents endothelial damage and liver congestion in a mouse model of heme overload." in: The American journal of pathology, Vol. 173, Issue 1, pp. 289-99, 2008 (PubMed).

Nedredal, Amiot, Nyberg et al.: "Optimization of mass transfer for toxin removal and immunoprotection of hepatocytes in a bioartificial liver." in: Biotechnology and bioengineering, Vol. 104, Issue 5, pp. 995-1003, 2009 (PubMed).

Beppu, Niwano, Tsukui et al.: "Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice." in: The Journal of toxicological sciences, Vol. 34, Issue 5, pp. 501-10, 2009 (PubMed).

Okura, Komoda, Saga et al.: "Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells." in: Tissue engineering. Part C, Methods, Vol. 16, Issue 4, pp. 761-70, 2010 (PubMed).

Hyvelin, Maurel, Uzbekov et al.: "Hemin prevents in-stent stenosis in rat and rabbit models by inducing heme-oxygenase-1." in: Journal of vascular surgery, Vol. 51, Issue 2, pp. 417-28, 2010 (PubMed).

Holt, Yin, Ju: "Exacerbation of acetaminophen-induced disturbances of liver sinusoidal endothelial cells in the absence of Kupffer cells in mice." in: Toxicology letters, Vol. 194, Issue 1-2, pp. 34-41, 2010 (PubMed).

Akachi, Shiina, Kawaguchi et al.: "1-methylmalate from camu-camu (Myrciaria dubia) suppressed D-galactosamine-induced liver injury in rats." in: Bioscience, biotechnology, and biochemistry, Vol. 74, Issue 3, pp. 573-8, 2010 (PubMed).

Zhang, Oyesanya, Campbell et al.: "Preclinical assessment of simultaneous targeting of epidermal growth factor receptor (ErbB1) and ErbB2 as a strategy for cholangiocarcinoma therapy." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 3, pp. 975-86, 2010 (PubMed).

Vera, Stec: "Moderate hyperbilirubinemia improves renal hemodynamics in ANG II-dependent hypertension." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 299, Issue 4, pp. R1044-9, 2010 (PubMed).

Park, Chen, Kim et al.: "Cytokines induce small intestine and liver injury after renal ischemia or nephrectomy." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 91, Issue 1, pp. 63-84, 2010 (PubMed).

Siciliano, Malpeli, Platt et al.: "Abnormal modulation of cell protective systems in response to ischemic/reperfusion injury is important in the development of mouse sickle cell hepatopathy." in: Haematologica, Vol. 96, Issue 1, pp. 24-32, 2011 (PubMed).

Fu, Kang, Ahn et al.: "Hyperbilirubinemia reduces the streptozotocin-induced pancreatic damage through attenuating the oxidative stress in the Gunn rat." in: The Tohoku journal of experimental medicine, Vol. 222, Issue 4, pp. 265-73, 2010 (PubMed).

Sekiya, Suzuki: "Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors." in: Nature, Vol. 475, Issue 7356, pp. 390-3, 2011 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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