NSF ELISA Kit (N-Ethylmaleimide-Sensitive Factor)

Details for Product NSF ELISA Kit No. ABIN2044029, Supplier: Log in to see
Antigen
  • NSF
  • 18.m06598
  • N-ethylmaleimide sensitive factor
  • T1J1.4
  • T1J1_4
  • AI316878
  • AU020090
  • AU067812
  • SKD2
  • nsf
  • si:bz18k17.1
  • wu:fj33g11
  • N-ethylmaleimide-sensitive factor
  • N-ethylmaleimide sensitive factor
  • N-ethylmaleimide sensitive fusion protein
  • N-ethylmaleimide-sensitive factor a
  • NSF
  • nsf
  • BBOV_II007200
  • TGME49_118510
  • Nsf
  • nsfa
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
6
1
1
Method Type
Sandwich ELISA
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.116 ng/mL
Alternative Name N-Ethylmaleimide Sensitive Factor (NSF) (NSF ELISA Kit Abstract)
Plate Pre-coated
Protocol N-Ethylmaleimide Sensitive Factor (NSF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
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