20-HETE ELISA Kit

Catalog No. ABIN614165

Product Details

Target: 20-HETE

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Purpose: Competitive ELISA for hypertension

Analytical Method: Quantitative

Specificity: Anti-20-HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid, linoleic acid and linolenic acid as shown in competitive ELISA analysis.

Sensitivity: 10 pg/mL

Characteristics: This competitive ELISA kit is for determination of 20-HETE (also known as 20-OH-AA) levels in biological samples. The specificity of the 20-HETE ELISA was investigated using authentic 20-HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20-HETE for binding to antibodies for 20-HETE. Anti-20-HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no crossreactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20-HETE and AA is an oxygen molecule, the specificity of this 20-HETE ELISA is a surprise.

Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20-HETE synthesis) activity. Increased circulating insulin inhibits 20- HETE synthesis in obese hypertensive subjects. Recently, CYP4F2 genetic variants, which increased urinary 20-HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population.

This kit can be used for the determination of 20-HETE in serum, plasma, cells, and tissues following proper isolation and purification.

Components: Antibodies for this Kit were raised in Goat.

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Tunaru, Bonnavion, Brandenburger, Preussner, Thomas, Scholich, Offermanns: "20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1." in: Nature communications, Vol. 9, Issue 1, pp. 177, (2018) (PubMed).

Costa, Ceravolo, Echem, Hashimoto, Costa, Santos-Eichler, Oliveira, Jiménez-Altayó, Akamine, Dantas, Carvalho et al.: "Detrimental Effects of Testosterone Addition to Estrogen Therapy Involve Cytochrome P-450-Induced 20-HETE Synthesis in Aorta of Ovariectomized Spontaneously Hypertensive Rat (SHR), a Model of ..." in: Frontiers in physiology, Vol. 9, pp. 490, (2018) (PubMed).

Cuez, Korkmaz, Buharalioglu, Sahan-Firat, Falck, Malik, Tunctan: "A synthetic analogue of 20-HETE, 5,14-HEDGE, reverses endotoxin-induced hypotension via increased 20-HETE levels associated with decreased iNOS protein expression and vasodilator prostanoid production in rats." in: Basic & clinical pharmacology & toxicology, Vol. 106, Issue 5, pp. 378-88, (2010) (PubMed).

Sahan-Firat, Jennings, Yaghini, Song, Estes, Fang, Farjana, Khan, Malik: "2,3',4,5'-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1." in: American journal of physiology. Heart and circulatory physiology, Vol. 299, Issue 6, pp. H1891-901, (2010) (PubMed).

Tunctan, Korkmaz, Cuez, Kemal Buharalioglu, Sahan-Firat, Falck, Malik: "Contribution of Vasoactive Eicosanoids and Nitric Oxide Production to the Effect of Selective Cyclooxygenase-2 Inhibitor, NS-398, on Endotoxin-Induced Hypotension in Rats." in: Basic & clinical pharmacology & toxicology, (2010) (PubMed).

Imaizumi, Grijalva, Navab, Van Lenten, Wagner, Anantharamiah, Fogelman, Reddy: "L-4F differentially alters plasma levels of oxidized fatty acids resulting in more anti-inflammatory HDL in mice." in: Drug metabolism letters, Vol. 4, Issue 3, pp. 139-48, (2010) (PubMed).

Certíková Chábová, Walkowska, Kompanowska-Jezierska, Sadowski, Kujal, Vernerová, Vanourková, Kopkan, Kramer, Falck, Imig, Hammock, Vanecková, Cervenka: "Combined inhibition of 20-hydroxyeicosatetraenoic acid formation and of epoxyeicosatrienoic acids degradation attenuates hypertension and hypertension-induced end-organ damage in Ren-2 transgenic rats." in: Clinical science (London, England : 1979), Vol. 118, Issue 10, pp. 617-32, (2010) (PubMed).

Tornavaca, Pascual, Barreiro, Grande, Carretero, Riera, Garcia-Arumi, Bardaji, González-Núñez, Montero, López-Novoa, Meseguer: "Kidney androgen-regulated protein transgenic mice show hypertension and renal alterations mediated by oxidative stress." in: Circulation, Vol. 119, Issue 14, pp. 1908-17, (2009) (PubMed).

Dołegowska, Błogowski, Domański: "Is it possible to predict the early post-transplant allograft function using 20-HETE measurements? A preliminary report." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 22, Issue 5, pp. 546-53, (2009) (PubMed).

Liu, Zhao, Wang, Yang, Zheng, Zhang, Chen, Liu: "Overexpression of cytochrome P450 4F2 in mice increases 20-hydroxyeicosatetraenoic acid production and arterial blood pressure." in: Kidney international, Vol. 75, Issue 12, pp. 1288-96, (2009) (PubMed).

Chen, Li, Liao, Wu, Liu, Ma, Zhou, Elbekai, Edin, Zeldin, Wang: "Selective inhibitors of CYP2J2 related to terfenadine exhibit strong activity against human cancers in vitro and in vivo." in: The Journal of pharmacology and experimental therapeutics, Vol. 329, Issue 3, pp. 908-18, (2009) (PubMed).

Liu, Zhao, Nie, Shi, Fu, Li, Yu, Lu: "Association of a functional cytochrome P450 4F2 haplotype with urinary 20-HETE and hypertension." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 4, pp. 714-21, (2008) (PubMed).

Target Details

Target: 20-HETE