Annexin V-Alexa488 protein (Alexa Fluor 488)

Details for Product No. ABIN1047952
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Protein Name
Mouse (Murine)
Protein Type Recombinant
Purification tag / Conjugate
Alexa Fluor 488
Pubmed 1 reference available
Catalog no. ABIN1047952
Quantity 50 µL
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Characteristics Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5
Material not included Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml
Gene ID 11747
UniProt P48036
Application Notes 5 l are suitable for one assay
Assay Procedure Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Storage 4 °C
Product cited in: Vermes, Haanen, Steffens-Nakken et al.: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, 1995 (PubMed).

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