Annexin V-Alexa488 protein (Alexa Fluor 488)

Name: Annexin V-Alexa488

Protein Type: Recombinant

Host: Please enquire

Origin (Gene): Mouse (Murine)

Conjugate: Alexa Fluor 488

Catalog no.: ABIN1047952

Additional Information

Gene ID: 11747

UniProt: P48036

Characteristics: Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5

Application Details

Assay Procedure: Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).

Application Notes: 5 l are suitable for one assay

Material not included: Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml

Preservative: 0.02% Sodium azide

Storage: 2-8°C (in the dark, avoid exposure to light)

Restrictions: For Research Use only

Publications

Product: Vermes, Haanen, Steffens-Nakken et al.: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, 1995 (PubMed).