Human Herpesvirus Type 2 ICP34.5 (N-Term) antibody

Catalog No. ABIN966331

This Rabbit Polyclonal antibody specifically detects Human Herpesvirus Type 2 ICP34.5 in IHC. It exhibits reactivity toward Herpes Simplex Virus (HSV).It has been mentioned in 1 publication

Quick Overview for Human Herpesvirus Type 2 ICP34.5 (N-Term) antibody (ABIN966331)

Target: Human Herpesvirus Type 2 ICP34.5

Reactivity: Herpes Simplex Virus (HSV)

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC)

Product Details

Binding Specificity: N-Term

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to near N-terminal residues of Human herpesvirus 2 ICP34.5 (Infected cell protein 34.5)

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Dolan, Jamieson, Cunningham, Barnett, McGeoch: "The genome sequence of herpes simplex virus type 2." in: Journal of virology, Vol. 72, Issue 3, pp. 2010-21, (1998) (PubMed).

Target Details

Target: Human Herpesvirus Type 2 ICP34.5

Target Type: Viral Protein

Background: ICP34.5 (Infected cell protein 34.5) contributes to HSV resistance to the antiviral effects of alpha/beta interferon. ICP34.5 (Infected cell protein 34.5) recruits the serine/threonineprotein phosphatase PPP1CA/PP1-alpha to dephosphorylate the translation initiation factor eIF-2A, thereby couteracting the host shutoff of protein synthesis involving double-stranded RNA-dependent protein kinase EIF2AK2/PKR. ICP34.5 also downmodulates the host MHC class II proteins cell surface expression. ICP34.5 acts as a neurovirulence factor that has a profound effect on the growth of the virus in central nervous system tissue, probably through its ability to maintain an environement favorable for viral replication. ICP34.5 interacts with human PPP1CA to form a high-molecular-weight complex that dephosphorylates eIF2-alpha. ICP34.5 binds to proliferating cell nuclear antigen (PCNA), which may release host cells from growth arrest and facilitate viral replication. At early times in infection, ICP34.5 colocalizes with PCNA and replication proteins in cell nuclei, before accumulating in the cytoplasm by 8 to 12 hours post-infection. The effects on the host cell are probably mediated by de novo-synthesized ICP34.5, the virion-derived population being either nonfunctional or present in very low amounts. ICP34.5 is detected as early as 3 hpi prior to viral replication but reaches maximal levels late in infection. ICP34.5 gene is therefore classified as gamma-1 or leaky late gene
Synonyms: RL1, Neurovirulence factor ICP34.5, protein gamma(1)34.5