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anti-Bromodomain Containing 4 (BRD4) Antibodies

The protein encoded by BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Additionally we are shipping BRD4 Kits (5) and BRD4 Proteins (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
BRD4 23476 O60885
BRD4 57261 Q9ESU6
BRD4 362844  
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Top anti-BRD4 Antibodies at

Showing 10 out of 90 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated ChIP, IF, WB Sample Type: HeLa Primary Antibody Dilution: 4 µg/mL Secondary Antibody: Anti-rabbit Alexa 546 Secondary Antibody Dilution:  µg/mL   Gene Name: BRD4 Chromatin Immunoprecipitation (ChIP) Using BRD4 Antibody - C-terminal region (ARP39076_P050) and HCT116 Cells 100 μL Log in to see 2 to 3 Days
Human Rabbit Un-conjugated ChIP, WB BRD4 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 10 µg of chromatin from TNF-α treated HT-29 colorectal adenocarcinoma cells and 5 µl BRD4 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. BRD4 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (30 µg) probed with BRD4 antibody (pAb) at a dilution of 1:500. 100 μL Log in to see 1 to 2 Days
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, PLA, WB Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH. 100 μL Log in to see 5 to 7 Days
Human Rabbit Un-conjugated WB Host: Rabbit  Target Name: Brd4  Sample Tissue: Rat Small Intestine lysates  Antibody Dilution: 1.0 µg/mL 100 μL Log in to see 2 to 3 Days
Human Rabbit Un-conjugated WB 100 μg Log in to see 2 to 3 Days
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), SimWes Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s). Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli. 0.1 mL Log in to see 8 to 11 Days
Human Rabbit Un-conjugated WB 100 μg Log in to see 2 to 3 Days
Human Rabbit Un-conjugated WB BRD4 Antibody (C-term)  western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the BRD4 antibody detected the BRD4 protein (arrow). 400 μL Log in to see 10 to 11 Days
Human Rabbit Un-conjugated WB Western blot of BRD4 antibody . Lane 1: BRD4 (342-460aa) 10 ng. Lane 2: BRD4 (342-460aa) 150 ng. Lane 2: BRD4 (342-460aa) 50 ng. 100 μg Log in to see 2 to 3 Days
Human Rabbit Un-conjugated IHC (p), WB Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with BRD4 antibody ABIN214729 at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. 50 μg Log in to see 8 to 10 Days

BRD4 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Human , , ,
, , , , ,
Mouse (Murine)

Rat (Rattus)

Top referenced anti-BRD4 Antibodies

  1. Human Polyclonal BRD4 Primary Antibody for ICC, IF - ABIN438710 : Rahman, Sowa, Ottinger, Smith, Shi, Harper, Howley: The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. in Molecular and cellular biology 2011 (PubMed)
    Show all 3 references for ABIN438710

  2. Human Polyclonal BRD4 Primary Antibody for ICC, IF - ABIN4285227 : Zuber, Shi, Wang, Rappaport, Herrmann, Sison, Magoon, Qi, Blatt, Wunderlich, Taylor, Johns, Chicas, Mulloy, Kogan, Brown, Valent, Bradner, Lowe, Vakoc: RNAi screen identifies Brd4 as a therapeutic target in acute myeloid leukaemia. in Nature 2011 (PubMed)
    Show all 2 references for ABIN4285227

  3. Human Polyclonal BRD4 Primary Antibody for ChIP, WB - ABIN2668492 : Flajollet, Rachez, Ploton, Schulz, Gallais, Métivier, Pawlak, Leray, Issulahi, Héliot, Staels, Salbert, Lefebvre: The elongation complex components BRD4 and MLLT3/AF9 are transcriptional coactivators of nuclear retinoid receptors. in PLoS ONE 2013 (PubMed)

  4. Cow (Bovine) Polyclonal BRD4 Primary Antibody for ChIP, IF - ABIN2780921 : Schweiger, You, Howley: Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation function. in Journal of virology 2006 (PubMed)

More Antibodies against BRD4 Interaction Partners

Zebrafish Bromodomain Containing 4 (BRD4) interaction partners

  1. Brd4 associates with mitotic chromosomes throughout early zebrafish embryogenesis

Human Bromodomain Containing 4 (BRD4) interaction partners

  1. this study suggests that BRD4 is one of the major contributors to the invasion-prone phenotype of non-small cell lung cancer, and a potential therapeutic target of non-small cell lung cancer.

  2. This study demonstrated that the developed fluorescent probe will also serve as a powerful tool to evaluate BRD4 inhibitors in living cells.

  3. High BRD4 expression is associated with hepatocellular carcinoma.

  4. Ewing sarcoma may be susceptible to treatment with epigenetic inhibitors blocking BRD3 (show BRD3 Antibodies)/4 activity and the associated pathognomonic EWS (show EWSR1 Antibodies)-FLT1 (show FLT1 Antibodies) transcriptional program.

  5. HPV16 E2-Brd4 interaction is more responsible for the transcriptional activation of host genes rather than repression.

  6. combined administration of lenalidomide and BRD4 inhibitor JQ-1 significantly increased the survival of PEL bearing NOD-SCID (show PRKDC Antibodies) mice in an orthotopic xenograft model

  7. findings identify key structural features of the ET domain of Brd4 that allow for interactions with both cellular and viral proteins.

  8. we found that NF-kappaB (show NFKB1 Antibodies), BRD4 and RNA POL II were rapidly distributed at the upstream regions of miR (show MLXIP Antibodies)-146a and miR (show MLXIP Antibodies)-155, and more importantly mediated the formation of the super enhancers that drive miR (show MLXIP Antibodies)-146a and miR (show MLXIP Antibodies)-155 transcription.

  9. In conclusion, our findings revealed that the aberrant expression of BRD4 in thyroid cancer is possibly involved in tumor progression, and JQ1 is potentially an effective chemotherapeutic agent against human thyroid cancer.

  10. novel actions of BRD4 and of NELF-E (show RDBP Antibodies) in GR-controlled gene induction have been uncovered.

Mouse (Murine) Bromodomain Containing 4 (BRD4) interaction partners

  1. the interaction between BRD4 and Mediator has functional importance for gene-specific transcriptional activation and for acute myeloid leukemia (show BCL11A Antibodies) maintenance.

  2. our data demonstrate the fundamental role of Brd4 in monitoring cell differentiation through its interaction with acetylated histone marks and disruption of Brd4 may cause aberrant differentiation.

  3. Data show that CCAAT-enhancer-binding protein-alpha (C/EBPalpha (show CEBPA Antibodies)) directly regulates Kruppel-like factor 4 (Klf4 (show KLF4 Antibodies)) expression and increasing the levels of histone demethylase (show MBD2 Antibodies) Lsd1 (show KDM1A Antibodies) and transcription factor Brd4 in B cell.

  4. BRD4 can act as a global genomic regulator to direct the fibrotic response through its coordinated regulation of myofibroblast transcription.

  5. BRD4 is critical for the maintenance of ESC pluripotency and that this occurs primarily through the maintenance of Nanog expression.

  6. The BET family member BRD4 interacts with OCT4 (show POU5F1 Antibodies) and regulates pluripotency gene expression

  7. Brd4 provides a critical link between neuronal activation and the transcriptional responses that occur during memory formation

  8. Aire's collaboration with the bromodomain-containing protein, Brd4, uncovered correspondence between those genes induced by Aire (show AIRE Antibodies) and those inhibited by a small-molecule bromodomain blocker.

  9. Data suggest that Brd4 is essential for Toll (show TLR4 Antibodies)-like receptor-stimulated interferon-beta (IFNB (show IFNB1 Antibodies)) gene transcription by permitting transcription factors to interact with the IFNB (show IFNB1 Antibodies) promoter in macrophages.

  10. Data show that hematopoietic transcription factors (TFs) promote bromodomain containing 4 protein (BRD4) recruitment to their occupied sites.

BRD4 Antigen Profile

Protein Summary

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15\;19)(q13\;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Gene names and symbols associated with BRD4

  • bromodomain containing 4 (brd4) antibody
  • bromodomain containing 4 (BRD4) antibody
  • bromodomain containing 4 (Brd4) antibody
  • BRD4 antibody
  • brd4-b antibody
  • Brd5 antibody
  • CAP antibody
  • fb18g03 antibody
  • HUNK1 antibody
  • HUNKI antibody
  • MCAP antibody
  • WI-11513 antibody
  • wu:fb18g03 antibody

Protein level used designations for BRD4

Bromodomain-containing protein 4B , bromodomain-containing protein 4B , bromodomain containing 4 , bromodomain-containing protein 4 , bromodomain 4 , bromodomain-containing protein 4-like , bromodomain containing protein 4 , bromodomain-containing 4 , chromosome-associated protein , bromodomain-containing 5 , fsh-like protein , mitotic chromosome-associated protein

446381 Xenopus laevis
455794 Pan troglodytes
570531 Danio rerio
100062072 Equus caballus
100216259 Xenopus (Silurana) tropicalis
100409351 Callithrix jacchus
100463607 Ailuropoda melanoleuca
100074736 Ornithorhynchus anatinus
100352214 Oryctolagus cuniculus
100533540 Sus scrofa
100593625 Nomascus leucogenys
23476 Homo sapiens
484869 Canis lupus familiaris
614048 Bos taurus
57261 Mus musculus
362844 Rattus norvegicus
Selected quality suppliers for anti-BRD4 (BRD4) Antibodies
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