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CD8 antibody (CD8a Molecule)

Details for Product anti-CD8A Antibody No. ABIN1027698, Supplier: Log in to see
Antigen
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
Alternatives
anti-Rat (Rattus) CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Rat (Rattus)
758
399
128
68
28
27
25
20
20
16
15
12
12
11
11
10
10
9
8
8
3
3
2
2
2
2
1
1
1
Host
Mouse
952
386
160
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is un-conjugated
242
187
121
84
30
30
26
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1162
275
218
188
134
111
105
82
65
33
30
25
23
17
14
10
7
7
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen High Mw glycoproteins from rat thymocytes
Clone OX-8
Isotype IgG1
Specificity The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Pathways TCR Signaling
Application Notes Functional Application: blocking

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Abe, Urakami, Ostanin et al.: "Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts." in: PLoS ONE, Vol. 4, Issue 11, pp. e7840, 2009 (PubMed).

Pino, OSullivan-Murphy, Lidstone et al.: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009 (PubMed).

Katsumata, Harigai, Sugiura et al.: "Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3772-9, 2007 (PubMed).

Ishida, Usui, Yamashiro et al.: "VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 483-9, 2003 (PubMed).

Mitnacht, Bischof, Torres-Nagel et al.: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Torres-Nagel, Kraus, Brown et al.: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).