CD8a Molecule (CD8A) antibody

Details for Product No. ABIN1027698
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Antigen
Synonyms cd8, LOC100136450, BB154331, Ly-2, Ly-35, Ly-B, Lyt-2, zgc:136643, CD8, Leu2, MAL, p32, RHACD8-4
Reactivity
Rat (Rattus)
(462), (299), (91), (51), (46), (25), (22), (16), (12), (10), (8), (6), (5), (5), (4), (2), (2), (1), (1)
Host
Mouse
(559), (262), (160)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(148), (89), (80), (36), (26), (17), (17), (9), (8), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB)
(702), (177), (152), (138), (107), (92), (73), (52), (47), (40), (28), (15), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN1027698
246.84 $
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Immunogen High Mw glycoproteins from rat thymocytes
Clone OX-8
Isotype IgG1
Specificity The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD8A
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Application Notes Functional Application: blocking

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Pino, OSullivan-Murphy, Lidstone et al.: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009 (PubMed).

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