Microtubule Star (MTS) (C-Term) antibody

Details for Product No. ABIN1042659
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Antigen
Epitope
C-Term
(1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(1), (1), (1)
Host
Mouse
(1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunoprecipitation (IP)
(1), (1), (1)
Pubmed 6 references available
Catalog no. ABIN1042659
Quantity 0.1 mL
Price
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Immunogen A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe. The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast
Sequence KLH-C-ßA-RRTPDYFL-OMe
Clone 2A10
Isotype IgG1
Specificity Recognises specifically the C-terminally methylated form of PP2A C subunit
Cross-Reactivity (Details) Predicted to work with: Saccharomyces cerevisiae
Characteristics Myeloma fusion partners:
X63-Ag8.653
Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa)
Purification Protein G
Alternative Name PP2A C
Background Synonyms:
PP2A alpha antibody,PP2A C antibody,PP2A-alpha antibody,PP2AA_HUMAN antibody,PP2Ac antibody,PP2CA antibody,PPP2CA antibody,Protein phosphatase 2, catalytic subunit, alpha isoform antibody,Replication protein C antibody
UniProt P63331
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try, WB: 1,500.
Comment

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
anti-Microtubule Star (MTS) (C-Term) antibody anti-Microtubule Star (MTS) (C-Term) antibody
Product cited in: Fellner, Lackner, Hombauer et al.: "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo." in: Genes & development, Vol. 17, Issue 17, pp. 2138-50, 2003 (PubMed).

Sontag, Hladik, Montgomery et al.: "Downregulation of protein phosphatase 2A carboxyl methylation and methyltransferase may contribute to Alzheimer disease pathogenesis." in: Journal of neuropathology and experimental neurology, Vol. 63, Issue 10, pp. 1080-91, 2004 (PubMed).

Sontag, Nunbhakdi-Craig, Sontag et al.: "Protein phosphatase 2A methyltransferase links homocysteine metabolism with tau and amyloid precursor protein regulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2751-9, 2007 (PubMed).

Hombauer, Weismann, Mudrak et al.: "Generation of active protein phosphatase 2A is coupled to holoenzyme assembly." in: PLoS biology, Vol. 5, Issue 6, pp. e155, 2007 (PubMed).

Li, Huang, Liang et al.: "Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by α4 protein in HEK293 cells." in: International journal of biological sciences, Vol. 7, Issue 6, pp. 740-52, 2011 (PubMed).

Benziane, Björnholm, Pirkmajer et al.: "Activation of AMP-activated protein kinase stimulates Na+,K+-ATPase activity in skeletal muscle cells." in: The Journal of biological chemistry, Vol. 287, Issue 28, pp. 23451-63, 2012 (PubMed).

Hosts (1)
Reactivities (1), (1), (1)
Applications (1), (1), (1)
Epitopes (1)
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