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Details for Product No. ABIN1042659

Microtubule Star (MTS) (C-Term) antibody

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Antigen
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Mouse (Murine), Rat (Rattus), Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives Western Blotting (WB), Immunoprecipitation (IP)
Pubmed 6 references available
Catalog no. ABIN1042659
Quantity 0.1 mL
Price
576.05 $   Plus shipping costs $45.00
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Availability Will be delivered in 5 to 6 Business Days
Immunogen A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe. The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast
Myeloma Fusion Partners: X63-Ag8.653
Clone 2A10
Isotype IgG1
Specificity Recognises specifically the C-terminally methylated form of PP2A C subunit
Cross-Reactivity Yeast (Saccharomyces cerevisiae)
Characteristics Mouse Anti-PP2A alpha (methyl L309) [2A10]
Purification Protein G
Alternative Name PP2A C
Gene ID 24672
OMIM 176915
UniProt P63331
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try WB: 1500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa)
Comment

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Preservative Sodium azide
Product cited in: Fellner, Lackner, Hombauer et al.: "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo." in: Genes & development, Vol. 17, Issue 17, pp. 2138-50, 2003 (PubMed).

Sontag, Hladik, Montgomery et al.: "Downregulation of protein phosphatase 2A carboxyl methylation and methyltransferase may contribute to Alzheimer disease pathogenesis." in: Journal of neuropathology and experimental neurology, Vol. 63, Issue 10, pp. 1080-91, 2004 (PubMed).

Sontag, Nunbhakdi-Craig, Sontag et al.: "Protein phosphatase 2A methyltransferase links homocysteine metabolism with tau and amyloid precursor protein regulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2751-9, 2007 (PubMed).

Hombauer, Weismann, Mudrak et al.: "Generation of active protein phosphatase 2A is coupled to holoenzyme assembly." in: PLoS biology, Vol. 5, Issue 6, pp. e155, 2007 (PubMed).

Li, Huang, Liang et al.: "Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by α4 protein in HEK293 cells." in: International journal of biological sciences, Vol. 7, Issue 6, pp. 740-52, 2011 (PubMed).

Benziane, Björnholm, Pirkmajer et al.: "Activation of AMP-activated protein kinase stimulates Na+,K+-ATPase activity in skeletal muscle cells." in: The Journal of biological chemistry, Vol. 287, Issue 28, pp. 23451-63, 2012 (PubMed).

Alternatives for antigen "Microtubule Star (MTS)", type "Antibodies"
Hosts (7), (1)
Reactivities (8), (7), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applications (8), (3), (2), (2), (1), (1)
Epitopes (4), (1), (1), (1), (1), (1)
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