Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (PerCP)

Details for Product No. ABIN1043023
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Antigen
Synonyms Apn, pepN, SC8E4A.13, APN, CD13, GP150, LAP1, P150, PEPN, AP-M, AP-N, Cd13, Apm, KZP, Lap1, rAPN, APDE, p150
Reactivity
Human, Non-Human Primate
(191), (57), (7), (7), (6), (5), (3), (1)
Host
Mouse
(158), (47), (47), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
PerCP
(40), (21), (16), (11), (9), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
(181), (56), (45), (45), (39), (37), (24), (13), (4), (3), (3), (1), (1), (1)
Pubmed 6 references available
Quantity 100 tests
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Catalog No. ABIN1043023
421.08 $
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Immunogen Human AML cells
Clone WM15
Isotype IgG1
Specificity The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.HLDA III, WS Code M 213HLDA IV, WS Code M 44HLDA IV, WS Code M 209HLDA V, WS Code M MA191
Characteristics The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name ANPEP
Background CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Gene ID 290
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Comment

CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Bradstock, Favaloro, Kabral et al.: "Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells." in: Pathology, Vol. 17, Issue 3, pp. 392-9, 1986 (PubMed).

Bradstock, Favaloro, Kabral et al.: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, 1985 (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, 1993 (PubMed).

Tokuhara, Hattori, Ishida et al.: "Clinical significance of aminopeptidase N in non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 13, pp. 3971-8, 2006 (PubMed).

Petrovic, Schacke, Gahagan et al.: "CD13/APN regulates endothelial invasion and filopodia formation." in: Blood, Vol. 110, Issue 1, pp. 142-50, 2007 (PubMed).

Terauchi, Kajiyama, Shibata et al.: "Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells." in: BMC cancer, Vol. 7, pp. 140, 2007 (PubMed).

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