CD13 antibody (Alanyl (Membrane) Aminopeptidase)

Details for Product anti-ANPEP Antibody No. ABIN93961
Antigen
Reactivity
Human, Non-Human Primate
389
92
34
23
12
9
8
7
3
2
1
1
Host
Mouse
315
89
47
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD13 antibody is un-conjugated
60
55
31
14
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
298
124
87
86
82
76
68
39
33
28
9
5
4
4
2
2
1
Options
Immunogen Human AML cells
Clone WM15
Isotype IgG1
Specificity The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
HLDA III, WS Code M 213
HLDA IV, WS Code M 44
HLDA IV, WS Code M 209
HLDA V, WS Code M MA191
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD13 (ANPEP Antibody Abstract)
Background CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Pathways Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (ABIN93961) Surface staining of human peripheral blood leukocytes with anti-CD13 mouse monoclonal...
Product cited in: McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, 2013 (PubMed).

Maeda, Wakasawa, Shima, Tsuboi, Aizawa, Tamai: "Role of polyamines derived from arginine in differentiation and proliferation of human blood cells." in: Biological & pharmaceutical bulletin, Vol. 29, Issue 2, pp. 234-9, 2006 (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, 1993 (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Berndt, Musgrove: "Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells." in: Pathology, Vol. 17, Issue 3, pp. 392-9, 1986 (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Musgrove: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, 1985 (PubMed).