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Transportin 1 (TNPO1) antibody

Details for Product No. ABIN108620
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Antigen
Synonyms TNPO1, ATTRN1, F12A24.13, F12A24_13, TRANSPORTIN 1, transportin 1, DKFZp469O204, mip1, zgc:100858, JC310, MIP1, SIN1, SIN1b, SIN1g, IPO2, KPNB2, MIP, TRN, AU021749, D13Ertd688e, Kpnb2, Mip1, Znf667
Reactivity
Mouse (Murine), Rabbit, Human, Monkey
(46), (23), (20), (15), (13), (12), (3), (3), (1), (1), (1), (1)
Host
Mouse
(37), (11), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
(40), (24), (15), (10), (10), (9), (9), (8), (8), (3)
Pubmed 7 references available
Quantity 50 µL
Shipping to United States (Change)
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Catalog No. ABIN108620
504.24 $
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Immunogen Recombinant his tagged transportin1 (Human)
Clone D45
Isotype IgG1
Characteristics Myeloma fusion partners:
SP2/0
Detects a band of approximately 93 kDa (predicted molecular weight: 107 kDa).
Purification Protein A
Alternative Name Transportin 1
Background Synonyms:
Importin beta 2 antibody,IPO 2 antibody,IPO2 antibody,Karyopherin (importin) beta 2antibody,Karyopherin beta 2 antibody,KPN B2 antibody,KPNB 2 antibody,KPNB2 antibody,M9 interacting proteinantibody,M9 region interacting protein antibody,M9 region interaction protein antibody,M9-interacting proteinantibody,MIP 1 antibody,MIP antibody,MIP1 antibody,TNPO 1 antibody,TNPO1 antibody,Transportinantibody,Transportin-1 antibody,Transportin1 antibody,TRN antibody
UniProt Q8BFY9
Research Area Signaling
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try, Flow Cyt: Use 1µg for 106 cells. ICC/IF: Use a concentration of 5 µg/mL. IHC-P: Use a concentration of 1 µg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1:8000.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Supplier Images
anti-Transportin 1 (TNPO1) antibody Western blot for anti-Transportin1 on HeLa cell extracts
anti-Transportin 1 (TNPO1) antibody (2) anti-Transportin 1 (TNPO1) antibody (Image 2)
Product cited in: Siomi, Eder, Kataoka et al.: "Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1181-92, 1997 (PubMed).

Favre, Camps, Arod et al.: "Chemokine receptor CCR2 undergoes transportin1-dependent nuclear translocation." in: Proteomics, Vol. 8, Issue 21, pp. 4560-76, 2008 (PubMed).

Chen, Shao, Cobos et al.: "4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone [corrected] induces CRM1-dependent p53 nuclear accumulation in human bronchial epithelial cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 116, Issue 1, pp. 206-15, 2010 (PubMed).

Janiszewska, De Vito, Le Bitoux et al.: "Transportin regulates nuclear import of CD44." in: The Journal of biological chemistry, Vol. 285, Issue 40, pp. 30548-57, 2010 (PubMed).

Gu, Tsuji, Jarboui et al.: "Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import." in: Retrovirology, Vol. 8, pp. 17, 2011 (PubMed).

Brelstaff, Lashley, Holton et al.: "Transportin1: a marker of FTLD-FUS." in: Acta neuropathologica, Vol. 122, Issue 5, pp. 591-600, 2011 (PubMed).

Yu, Boyce, Wands et al.: "Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 13, pp. 4834-9, 2012 (PubMed).

Validation Images
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