Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody

Details for Product No. ABIN108727
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CEACAM5, CEA, CEACAM, RGD1311281, CD66e, 1600029H12Rik, Psg30
Reactivity
Human
(299), (9), (2)
Host
Mouse (Murine)
(206), (92), (8), (3), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (6), (6), (4)
Application
Flow Cytometry (FACS), Cell-ELISA (cELISA), ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(114), (110), (105), (68), (63), (42), (32), (23), (21), (19), (16), (13), (6), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 13 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 5 to 6 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN108727
450.12 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen immunisation with extracted protein of CEACAM5
Clone 26-3-13
Isotype IgG1
Specificity Anti-human CEACAM5 (CEA, CD66e)
Purification Protein G
Alternative Name CEACAM5
Background CEACAM5 or CEA is the name-giving member of the CEACAM family. CEACAM5 is used as a tumor marker for early detection of recurrent disease and is a potential target of tumor imaging, drugs and immunotargeting. 26/3/13 can be used to distinguish CEACAM5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules.
UniProt P06731
Research Area Cancer
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 Western Blot: 4 µg/mL
Immunohistology: 1-2 µg/10^6 cells (on cryosections)
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD66e, CEA

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody Specificity testing of 26/3/13. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody (2) FACS analysis of BOSC23 cells using 26/3/13. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/3/13 was detected witha PE conjugated sec
Product cited in: Saeland, Belo, Mongera et al.: "Differential glycosylation of MUC1 and CEACAM5 between normal mucosa and tumour tissue of colon cancer patients." in: International journal of cancer. Journal international du cancer, 2011 (PubMed).

Background publications Schmitter, Agerer, Peterson et al.: "Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens." in: The Journal of experimental medicine, Vol. 199, Issue 1, pp. 35-46, 2004 (PubMed).

Partyka, McDonald, Maupin et al.: "Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification." in: Journal of proteome research, Vol. 11, Issue 5, pp. 2904-11, 2012 (PubMed).

Peng, Oberst, Huang et al.: "The CEA/CD3-bispecific antibody MEDI-565 (MT111) binds a nonlinear epitope in the full-length but not a short splice variant of CEA." in: PLoS ONE, Vol. 7, Issue 5, pp. e36412, 2012 (PubMed).

Drukker, Tang, Ardehali et al.: "Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells." in: Nature biotechnology, Vol. 30, Issue 6, pp. 531-42, 2012 (PubMed).

General Strobel, Strobel, Bross et al.: "Effects of human bone marrow stroma on the growth of human tumor cells." in: Cancer research, Vol. 49, Issue 4, pp. 1001-7, 1989 (PubMed).

Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).

Thompson, Mössinger, Reichardt et al.: "A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 311-9, 1993 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Schölzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Baczy?ska, Wietrzyk, Madej et al.: "The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression." in: Cellular & molecular biology letters, Vol. 8, Issue 2, pp. 471-86, 2003 (PubMed).

Kammerer, Riesenberg, Weiler et al.: "The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma." in: The Journal of pathology, Vol. 204, Issue 3, pp. 258-67, 2004 (PubMed).

Nöckel, van den Engel, Winter et al.: "Characterization of gastric adenocarcinoma cell lines established from CEA424/SV40 T antigen-transgenic mice with or without a human CEA transgene." in: BMC cancer, Vol. 6, pp. 57, 2006 (PubMed).

Validation Images
Did you look for something else?
back to top