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Biotin antibody (FITC)

FACS, IF Host: Goat Polyclonal FITC

Catalog No. ABIN1105535

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
63

38

30
Goat
Compatible Secondaries
Clonality
83

40

1
Polyclonal
Conjugate
35

14

8

8

8

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to FITC
Application
78

56

51

47

39

33

16

13

11

9

6

5

4

4

3

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics

Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 4.6 Moles FITC per mole of Goat IgG.

Purification

Immunoaffinity chromatography.

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM Host: Mouse Monoclonal 4C7-D5 unconjugated

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody
WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody (DyLight 488)
WB, FACS, IF, FM, FLISA, DB, MA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
ELISA, FACS Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, ELISA, FACS, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
ELISA, FACS, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal B-E24 unconjugated

anti-Biotin antibody
Reactivity: Human WB Host: Rabbit Monoclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Reconstitution

Restore with 1.0 mL of deionized water (or equivalent).

Concentration

1.0 mg/mL (by UV absorbance at 280 nm)

Buffer

0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage

4 °C/-20 °C

Storage Comment

Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.