Biotin antibody

Catalog No. ABIN6941338

The Mouse Monoclonal anti-Biotin antibody (Clone Hyb-8) (ABIN6941338) specifically detects Biotin in WB, IHC, FACS and IF. The antibody is reactive with Various Species samples.

Quick Overview for Biotin antibody (ABIN6941338)

Target: Biotin

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: Hyb-8

Product Details anti-Biotin Antibody

Purpose: Biotin (Vitamin B7 or Vitamin H) Antibody

Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Biotinylated sheep immunoglobulin

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Biotinylated proteins in solution or on tissues.

Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Biotin

Target: Biotin

Alternative Name: Biotin (Vitamin B7 or Vitamin H)

Target Type: Chemical

Background:

Vitamin B7 or Vitamin H

Biotinylated proteins in solution or on tissues.

Molecular Weight: 244Da