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Biotin antibody

Name: Biotin antibody
Brand: antibodies-online
SKU: ABIN6941338
Price: 597.38 USD
Availability: InStock

WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

Formalin-fixed, paraffin-embedded human Tonsil stained with Biotinylated Lambda Light Chain probe followed by Anti-Biotin Mouse Monoclonal Antibody (Hyb-8).

IHC

SDS-PAGE Analysis of Purified anti-Biotin Mouse Monoclonal antibody (Hyb-8).

SDS

2 images

Catalog No. ABIN6941338

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
57

35

29
Mouse
Compatible Secondaries
Clonality
77

37
Monoclonal
Conjugate
30

11

8

8

7

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is un-conjugated
Application
58

48

46

32

31

19

14

12

12

12

9

9

7

5

4

4

3

2

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)

Specificity

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Purification

Purified by Protein A/G

Immunogen

Biotinylated sheep immunoglobulin

Clone

Hyb-8

Isotype

IgG1 kappa
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WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal B-E24 unconjugated

Application Notes

Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date

24 months
Target

Biotin

Alternative Name

Biotin (Vitamin B7 or Vitamin H) (Biotin Products)

Target Type

Chemical

Molecular Weight

244Da