FAS antibody

Catalog No. ABIN1106610

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes the human Fas antigen specifically.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulfate precipitation and affinity chromatography on protein A agarose

Immunogen: Recombinant human Fas

Clone: UB2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water.

Buffer: PBS ( pH 7.2), 0.09 % NaN3, 1 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

References for anti-FAS antibody (ABIN1106610)

Mezosi, Wang, Utsugi, Bajnok, Bretz, Gauger, Thompson, Baker: "Induction and regulation of Fas-mediated apoptosis in human thyroid epithelial cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 3, pp. 804-11, (2005) (PubMed).

Hauptschein, Sloan, Torella, Moezzifard, Giel-Moloney, Zehetmeier, Unger, Ilag, Jay: "Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptosis." in: Cancer research, Vol. 65, Issue 5, pp. 1887-96, (2005) (PubMed).

Suhara, Fukuo, Yasuda, Tsubakimoto, Takemura, Kawamoto, Yokoi, Mogi, Kaimoto, Ogihara: "Homocysteine enhances endothelial apoptosis via upregulation of Fas-mediated pathways." in: Hypertension, Vol. 43, Issue 6, pp. 1208-13, (2004) (PubMed).

Suhara, Kim, Kirshenbaum, Walsh: "Suppression of Akt signaling induces Fas ligand expression: involvement of caspase and Jun kinase activation in Akt-mediated Fas ligand regulation." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 680-91, (2001) (PubMed).

Shinohara, Yagita, Ikawa, Oyaizu: "Fas drives cell cycle progression in glioma cells via extracellular signal-regulated kinase activation." in: Cancer research, Vol. 60, Issue 6, pp. 1766-72, (2000) (PubMed).

Sata, Suhara, Walsh: "Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 2, pp. 309-16, (2000) (PubMed).

Hiromatsu, Hoshino, Yagita, Koga, Sakisaka, Honda, Yang, Kayagaki, Okumura, Nonaka: "Functional Fas ligand expression in thyrocytes from patients with Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 84, Issue 8, pp. 2896-902, (1999) (PubMed).

Kuwano, Miyazaki, Hagimoto, Kawasaki, Fujita, Kunitake, Kaneko, Hara: "The involvement of Fas-Fas ligand pathway in fibrosing lung diseases." in: American journal of respiratory cell and molecular biology, Vol. 20, Issue 1, pp. 53-60, (1999) (PubMed).

Dai, Price, Brunner, Krantz: "Fas ligand is present in human erythroid colony-forming cells and interacts with Fas induced by interferon gamma to produce erythroid cell apoptosis." in: Blood, Vol. 91, Issue 4, pp. 1235-42, (1998) (PubMed).

Ando, Hiroishi, Kaneko, Moriyama, Muto, Kayagaki, Yagita, Okumura, Imawari: "Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5283-91, (1997) (PubMed).

Boirivant, Pica, DeMaria, Testi, Pallone, Strober: "Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis." in: The Journal of clinical investigation, Vol. 98, Issue 11, pp. 2616-22, (1997) (PubMed).

Hata, Matsuzaki, Takeya, Yoshida, Sonoki, Nagasaki, Kuribayashi, Kawano, Takatsuki: "Expression of Fas/Apo-1 (CD95) and apoptosis in tumor cells from patients with plasma cell disorders." in: Blood, Vol. 86, Issue 5, pp. 1939-45, (1995) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Background: It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

Gene ID: 355

NCBI Accession: NP_000034

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity