anti-EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN111178
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Antigen
Synonyms
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5 ... show more
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TROP1, CD326, EGP, Egp314, Ep-CAM, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40 show less
Reactivity
Human
(294), (49), (33)
Host
Mouse
(217), (83), (16), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
(27), (15), (11), (7), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(192), (114), (111), (86), (66), (61), (49), (48), (34), (11), (7), (5), (5), (5), (4), (3), (2), (1), (1)
Pubmed 9 references available
Quantity 50 μg
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Immunogen HT-29 colon carcinoma cell line
Clone HEA125
Isotype IgG1
Specificity Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Alternative Name CD326 / EPCAM / TACSTD1 (EPCAM Antibody Abstract)
Background The epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34) is widely expressed on cells of epithelial origin and tumors derived therefrom.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Application Notes Immunoblotting. Cell sorting. Cytological material. Immunofluorescence microscopy. Immunohistochemistry on frozen and paraffin-embedded tissue. With paraffin embeddedsections, protease pre-treatment is required prior to antibody application. Dilute at least 1: 10 with PBS, pH 7.4, for immunohistochemical application. Incubationtime: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Background publications Bechtel, Petty, Saccomanno: "Five year survival and later outcome of patients with X-ray occult lung cancer detected by sputum cytology." in: Lung cancer (Amsterdam, Netherlands), Vol. 30, Issue 1, pp. 1-7, 2005 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Hamburger, White, Dunn: "Secretion of transforming growth factors by primary human tumour cells." in: British journal of cancer, Vol. 51, Issue 1, pp. 9-14, 1985 (PubMed).

Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]." in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Harris: "Mutant p53--the commonest genetic abnormality in human cancer?" in: The Journal of pathology, Vol. 162, Issue 1, pp. 5-6, 1990 (PubMed).

General Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Validation Images
Catalog No. ABIN111178
368.50 $
Plus shipping costs $45.00
Quantity
Price
50 μg
368.50 $

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