Epithelial Cell Adhesion Molecule (EPCAM) antibody

Details for Product No. ABIN111178
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Antigen
Synonyms
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5 ... show more
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TROP1, CD326, EGP, Egp314, Ep-CAM, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40 show less
Reactivity
Human
(254), (64), (40), (17), (17), (17)
Host
Mouse
(175), (91), (13), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(25), (16), (10), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(144), (120), (97), (52), (52), (47), (44), (43), (30), (20), (5), (3), (2), (2), (1)
Pubmed 9 references available
Quantity 50 μg
Shipping to United States (Change)
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Catalog No. ABIN111178
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Immunogen HT-29 colon carcinoma cell line
Clone HEA125
Isotype IgG1
Specificity Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Species Reactivity: Tested: Human.
Purification Affinity Chromatography on Protein A
Alternative Name CD326 / EPCAM / TACSTD1
Background The epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34) is widely expressed on cells of epithelial origin and tumors derived therefrom.
Alternate names: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Application Notes Immunoblotting. Cell sorting. Cytological material. Immunofluorescence microscopy. Immunohistochemistry on frozen and paraffin-embedded tissue. With paraffin embeddedsections, protease pre-treatment is required prior to antibody application. Dilute at least 1: 10 with PBS, pH 7.4, for immunohistochemical application. Incubationtime: 1 h at RT, extended with paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4 0.09% Sodium Azide 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C.
Expiry Date 12 months
Background publications Harris: "Mutant p53--the commonest genetic abnormality in human cancer?" in: The Journal of pathology, Vol. 162, Issue 1, pp. 5-6, 1990 (PubMed).

Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]." in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Hamburger, White, Dunn: "Secretion of transforming growth factors by primary human tumour cells." in: British journal of cancer, Vol. 51, Issue 1, pp. 9-14, 1985 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Bechtel, Petty, Saccomanno: "Five year survival and later outcome of patients with X-ray occult lung cancer detected by sputum cytology." in: Lung cancer (Amsterdam, Netherlands), Vol. 30, Issue 1, pp. 1-7, 2005 (PubMed).

General Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

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