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EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN111178, Supplier: Login to see
Antigen
  • sb:cb6
  • tacstd
  • wu:fj17g02
  • zgc:110304
  • zgc:77119
  • tacstd1
  • MGC80540
  • EPCAM
  • TACSTD1
  • egp
  • ksa
  • m4s1
  • mk-1
  • cd326
  • egp40
  • mic18
  • trop1
  • ep-cam
  • hegp-2
  • co17-1a
  • ga733-2
  • GP40
  • LEU-9
  • TP41
  • Tp40
  • DIAR5
  • EGP-2
  • EGP314
  • EGP40
  • ESA
  • HNPCC8
  • KS1/4
  • KSA
  • M4S1
  • MIC18
  • MK-1
  • TROP1
  • CD326
  • EGP
  • Egp314
  • Ep-CAM
  • EpCAM1
  • GA733-2
  • Ly74
  • Tacsd1
  • Tacstd1
  • gp40
Reactivity
Human
456
94
45
2
1
1
1
Host
Mouse
353
128
40
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
36
24
17
13
6
5
5
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
301
220
137
136
125
119
74
55
47
13
8
5
5
4
4
4
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen HT-29 colon carcinoma cell line
Clone HEA125
Isotype IgG1
Specificity Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Alternative Name CD326 / EPCAM / TACSTD1 (EPCAM Antibody Abstract)
Background The epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34) is widely expressed on cells of epithelial origin and tumors derived therefrom.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Pathways
Application Notes Immunoblotting. Cell sorting. Cytological material. Immunofluorescence microscopy. Immunohistochemistry on frozen and paraffin-embedded tissue. With paraffin embeddedsections, protease pre-treatment is required prior to antibody application. Dilute at least 1: 10 with PBS, pH 7.4, for immunohistochemical application. Incubationtime: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Background publications Bechtel, Petty, Saccomanno: "Five year survival and later outcome of patients with X-ray occult lung cancer detected by sputum cytology." in: Lung cancer (Amsterdam, Netherlands), Vol. 30, Issue 1, pp. 1-7, 2005 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Hamburger, White, Dunn: "Secretion of transforming growth factors by primary human tumour cells." in: British journal of cancer, Vol. 51, Issue 1, pp. 9-14, 1985 (PubMed).

Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Hastka, Pfiester: "[Cell marker for the differential diagnosis of malignant mesotheliomas]." in: Der Pathologe, Vol. 9, Issue 4, pp. 245-7, 1989 (PubMed).

Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Harris: "Mutant p53--the commonest genetic abnormality in human cancer?" in: The Journal of pathology, Vol. 162, Issue 1, pp. 5-6, 1990 (PubMed).