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EpCAM antibody (AA 40-260)

This Rabbit Polyclonal antibody specifically detects EpCAM in WB and IF. It exhibits reactivity toward Human.It has been mentioned in 1 publication

IHC

Immunofluorescence analysis of HT-29 cells using EpCAM Rabbit pAb (ABIN3021637, ABIN3021638, ABIN3021639, ABIN1513585 and ABIN6215463) at dilution of 1:100 (40x lens). (EpCAM antibody (AA 40-260))

Western blot analysis of extracts of various cell lines, using TACSTD1 antibody. (EpCAM antibody (AA 40-260))

MTA1 could upregulate EpCAM expression and promote invasion and migration via upregulation of EpCAM expression in lung cancer cells. (EpCAM antibody (AA 40-260))

Expression of MTA1 and EpCAM in tissue microarray samples. (EpCAM antibody (AA 40-260))

IHC

Western blot analysis of extracts of various cell lines, using EpCAM antibody (ABIN3021637, ABIN3021638, ABIN3021639, ABIN1513585 and ABIN6215463) at 1:500 dilution. (EpCAM antibody (AA 40-260))

Immunofluorescence analysis of cells using EpCAM Rabbit pAb (ABIN3021637, ABIN3021638, ABIN3021639, ABIN1513585 and ABIN6215463) at dilution of 1:100 (40x lens). (EpCAM antibody (AA 40-260))

7 images

(1 reference)

Catalog No. ABIN3021638

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Quick Overview for EpCAM antibody (AA 40-260) (ABIN3021638)

Target

See all EpCAM (EPCAM) Antibodies

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This EpCAM antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF)

Binding Specificity
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AA 40-260

Sequence

VNNNRQCQCT SVGAQNTVIC SKLAAKCLVM KAEMNGSKLG RRAKPEGALQ NNDGLYDPDC DESGLFKAKQ CNGTSMCWCV NTAGVRRTDK DTEITCSERV RTYWIIIELK HKAREKPYDS KSLRTALQKE ITTRYQLDPK FITSILYENN VITIDLVQNS SQKTQNDVDI ADVAYYFEKD VKGESLFHSK KMDLTVNGEQ LDLDPGQTLI YYVDEKAPEF S

Cross-Reactivity

Human, Mouse, Rat

Characteristics

Polyclonal Antibodies

Purification

Affinity purification

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human EpCAM (NP_002345.2).

Isotype

IgG
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibody
EPCAM Reactivity: Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) antibody
EPCAM Reactivity: Human, Mouse WB, FACS, IHC, IF Host: Rabbit Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 289-314), (Cytoplasmic Domain) antibody
EPCAM Reactivity: Human, Cat, Dog IHC Host: Mouse Monoclonal EGP40-1110 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM Reactivity: Human WB, FACS, IHC, IP, ICC Host: Rabbit Monoclonal BLR192J unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibody
EPCAM Reactivity: Human, Mouse WB, FACS, IHC, IP, ICC, IF (p) Host: Rabbit Monoclonal BLR077G unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibody
EPCAM Reactivity: Human, Mouse WB, FACS, IHC, IP, ICC, IF (p) Host: Rabbit Monoclonal BLR077G unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibody
EPCAM Reactivity: Human, Cat, Dog, Guinea Pig WB, IHC, IF, ELISA Host: Mouse Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibody
EPCAM Reactivity: Human, Cat, Dog WB, FACS, IHC, IF, ELISA Host: Mouse Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibody
EPCAM Reactivity: Human WB, FACS, IHC, IF, ELISA Host: Mouse Monoclonal EGP40-1372 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibody
EPCAM Reactivity: Human, Cat, Dog WB, IHC Host: Mouse Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

Application Notes

WB,1:500 - 1:2000,IF,1:50 - 1:200

Restrictions

For Research Use only
Format

Liquid

Buffer

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid freeze / thaw cycles

Storage

-20 °C

Storage Comment

Store at -20°C. Avoid freeze / thaw cycles.
Zhou, Wang, Liu, Xue, Lin, Meng, Liang, Zhao, Liu, Qian: "MTA1-upregulated EpCAM is associated with metastatic behaviors and poor prognosis in lung cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 157, (2016) (PubMed).
Target

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name

EPCAM

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.,EPCAM,DIAR5,EGP-2,EGP314,EGP40,ESA,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK-1,TACSTD1,TROP1,Immunology & Inflammation,CD markers,EPCAM

Molecular Weight

34 kDa

Gene ID

4072

UniProt

P16422