Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (HEA125)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN111180

Additional Information

Characteristics: Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

Alternative name: CD326 / EPCAM / TACSTD1

Swiss-Prot: P16422

Immunogen: HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cellsof a mouse myeloma cell line.

Isotype: IgG1  (Matching secondary antibodies)

Clone: HEA125

Description: This antigen has been identified independently by a number of groups, and it has beenknown by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which havebeen described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by themajority of epithelial tissues, with the exception of adult squamous epithelium and somespecific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAMexpression has been reported to be a possible marker of early malignancy, with expressionbeing increased in tumour cells, and de novo expression being seen in dysplasticsquamous epithelium.

Specificity: This antibody recognises the 34kD cell surface antigen known as CD326 or Epithelial CellAdhesion Molecule (Ep-CAM). Species Reactivity: Tested: Human.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: Flow Cytometry: Use 5 µl of neat antibody to label 10e6 cells or 100ul cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1% pronase, 10 minutes. Recommended Positive Control: Human colon and appendix. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH 7.3 containing 0.09% Sodium Azide as preservative and 0.5% BSAas stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Publications: Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Momburg, Moldenhauer, Haemmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).