Epithelial Cell Adhesion Molecule (EPCAM) antibody

Details for Product No. ABIN111180
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DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:11030 ... show more
DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, EGP, Ep-CAM, CD326, Egp314, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40, sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2 show less
(223), (52), (39), (17), (17), (17), (2)
(173), (58), (12)
Clonality (Clone)
Monoclonal ()
(18), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(126), (114), (76), (50), (45), (39), (34), (34), (29), (20), (5), (5), (3), (2), (2), (2)
Pubmed 10 references available
Catalog no. ABIN111180
Quantity 0.1 mg
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Immunogen HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Clone HEA125
Isotype IgG1
Specificity This antibody recognises the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). Species Reactivity: Tested: Human.
Purification Affinity Chromatography
Alternative Name CD326 / EPCAM / TACSTD1
Background This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Alternate names: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345.2
UniProt P16422
Research Area Cancer
Application Notes Flow Cytometry: Use 5 µl of neat antibody to label 10e6 cells or 100ul cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1% pronase, 10 minutes. Recommended Positive Control: Human colon and appendix. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, pH 7.3 containing 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Kemmner, Moldenhauer, Schlag et al.: "Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads." in: Journal of immunological methods, Vol. 147, Issue 2, pp. 197-200, 1992 (PubMed).

Simon, Podolsky, Moldenhauer et al.: "Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2755-9, 1990 (PubMed).

Moldenhauer, Momburg, Möller et al.: "Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker." in: British journal of cancer, Vol. 56, Issue 6, pp. 714-21, 1988 (PubMed).

Edwards: "Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies." in: British journal of cancer, Vol. 51, Issue 2, pp. 149-60, 1985 (PubMed).

Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Winter, Nagtegaal, van Krieken et al.: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, 2003 (PubMed).

General Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Hosts (173), (58), (12)
Reactivities (223), (52), (39), (17), (17), (17), (2)
Applications (126), (114), (76), (50), (45), (39), (34), (34), (29), (20), (5), (5), (3), (2), (2), (2)
Conjugates (18), (8), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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