anti-EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN111180
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Antigen
Synonyms
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5 ... show more
sb:cb6, tacstd, wu:fj17g02, zgc:110304, zgc:77119, tacstd1, MGC80540, EPCAM, TACSTD1, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2, GP40, LEU-9, TP41, Tp40, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TROP1, CD326, EGP, Egp314, Ep-CAM, EpCAM1, GA733-2, Ly74, Tacsd1, Tacstd1, gp40 show less
Reactivity
Human
(299), (59), (42)
Host
Mouse
(214), (99), (17), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
(26), (15), (11), (7), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(199), (114), (111), (94), (71), (65), (52), (43), (34), (11), (7), (5), (5), (5), (5), (4), (2), (1), (1), (1)
Pubmed 10 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Immunogen HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Clone HEA125
Isotype IgG1
Specificity This antibody recognises the 34 kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography
Alternative Name CD326 / EPCAM / TACSTD1 (EPCAM Antibody Abstract)
Background This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Application Notes Flow Cytometry: Use 5 μL of neat antibody to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1 % pronase, 10 minutes. Recommended Positive Control: Human colon and appendix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.3 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Winter, Nagtegaal, van Krieken et al.: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, 2003 (PubMed).

Momburg, Moldenhauer, Hämmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Edwards: "Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies." in: British journal of cancer, Vol. 51, Issue 2, pp. 149-60, 1985 (PubMed).

Moldenhauer, Momburg, Möller et al.: "Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker." in: British journal of cancer, Vol. 56, Issue 6, pp. 714-21, 1988 (PubMed).

Simon, Podolsky, Moldenhauer et al.: "Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2755-9, 1990 (PubMed).

Kemmner, Moldenhauer, Schlag et al.: "Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads." in: Journal of immunological methods, Vol. 147, Issue 2, pp. 197-200, 1992 (PubMed).

General Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Racila, Euhus, Weiss et al.: "Detection and characterization of carcinoma cells in the blood." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4589-94, 1998 (PubMed).

Joplin, Strain, Neuberger: "Immuno-isolation and culture of biliary epithelial cells from normal human liver." in: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 25, Issue 12, pp. 1189-92, 1990 (PubMed).

Joplin, Strain, Neuberger: "Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: isolation, characterization, and short-term culture." in: The Journal of pathology, Vol. 162, Issue 3, pp. 255-60, 1991 (PubMed).

Validation Images
Catalog No. ABIN111180
1,116.50 $
Plus shipping costs $45.00
Quantity
Price
0.1 mg
1,116.50 $

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