Keratin 18 (KRT18) antibody

Details for Product No. ABIN111183
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Antigen
Synonyms krt18, MGC64569, MGC75922, KRT18, CYK18, K18, CK18, Krt1-18, DreK18, cb83, dapk1, sb:cb83, wu:fa13f02, wu:fb36b04, krtt1c6, zgc:66015
Reactivity
Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Trout, Zebrafish (Danio rerio)
(466), (109), (103), (42), (36), (34), (29), (13), (12), (7), (5), (5), (5), (4), (4), (3), (3), (2), (1), (1), (1)
Host
Mouse
(264), (232), (9), (4), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(31), (30), (8), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(363), (208), (150), (121), (121), (92), (91), (81), (50), (45), (37), (13), (10), (4), (3), (1), (1)
Pubmed 8 references available
Quantity 50 μg
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Catalog No. ABIN111183
390.50 $
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Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Clone Ks18-04
Isotype IgG1
Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish
Purification Protein A chromatography
Alternative Name Cytokeratin 18
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
NCBI Accession NP_000215
UniProt P05783
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Application Notes Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1.0 mL distilled water.
Buffer PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing). Dilute only prior to immediate use.
Storage -20 °C
Storage Comment Store the antiserum at -20 °C (aliquot contents to
Product cited in: Sharma, Cantz, Richter et al.: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, 2005 (PubMed).

Background publications Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Bantel, Ruck, Gregor et al.: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, 2001 (PubMed).

Bredt: "Nitric oxide signaling specificity--the heart of the problem." in: Journal of cell science, Vol. 116, Issue Pt 1, pp. 9-15, 2002 (PubMed).

Schaffeld, Knappe, Hunzinger et al.: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." in: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, 2003 (PubMed).

General Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Ikenouchi, Matsuda, Furuse et al.: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, 2003 (PubMed).

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