Keratin 18 (KRT18) antibody

Antigen: Keratin 18 (KRT18)

Synonyms: K18, CYK18, CK18, Krt1-18, MGC109143, cb83, dapk1, DreK18, sb:cb83, wu:fa13f02, wu:fb36b04, krt18, MGC64569, MGC75922, KRT18, MGC131121

Clonality: Monoclonal (Ks18-04)

Host: Mouse

Reactivity: Trout, Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111183

Additional Information

Alternative name: Cytokeratin 18

UniProt: P05783

Immunogen: Human keratin K18 from HeLa cytoskeletal preparation

Cross-Reactivity: Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)

Isotype: IgG1

Clone: Ks18-04

Description: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia andis present in a majority of adenocarcinomas and ductal carcinomas but not in squamouscell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies thatrecognize only Cytokeratins 8 and 18.

Characteristics: Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18

Application Details

Application Notes: Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS, pH 7.4, containing 0.5% BSA ans 0.09% sodium azideReconstitution: Restore in 1.0 ml distilled water.

Storage: Store the original vial at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (inaliquots)at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Publications

Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Bantel, Ruck, Gregor et al.: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, 2001 (PubMed).

Ikenouchi, Matsuda, Furuse et al.: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, 2003 (PubMed).