CD19 Molecule (CD19) antibody

Details for Product No. ABIN111246
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Antigen
Synonyms CD19, AW495831, B4, CVID3
Reactivity
Human
(422), (289), (46), (41), (41), (24), (24), (2)
Host
Mouse
(356), (149), (147), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(77), (60), (60), (42), (15), (11), (10), (10), (9), (8), (8), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Flow Cytometry (FACS)
(463), (103), (89), (67), (58), (58), (49), (42), (27), (26), (13), (1), (1), (1)
Pubmed 4 references available
Quantity 0.2 mg
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Catalog No. ABIN111246
467.50 $
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Immunogen SKLY 18 Lymphoma Cells. Remarks:
Clone J4-119
Isotype IgG1
Specificity J4.119 cross-reacts with B cells from Macaca fascicularis, Macaca mulatta and Papio cynocephalus . It reacts weakly with B cells from Rhesus monkey. The J4.119 MAb was assigned to the CD19 cluster for differentiation at the 4th International Workshop on Human Leucocyte Differentiation Antigens (HDLA) in Vienna, Austria (1989). Expression of the CD19 molecule is found on all B Lymphocytes, including pro-B cells, but is lost during maturation to plasma cells. The CD19 antigen is also expressed on the membrane of follicular dendritic cells and on most stabilized B cell lines. CD19 expression is not observed in normal T Lymphocytes, NK cells, Monocytes and Granulocytes. CD19 appears important in regulating B-cell activation and proliferation. In vitro studies show that the CD19 antibody has an inhibitory effect on the activation and proliferation of B Lymphocytes.
Purification Protein A Sepharose Chromatography.
Alternative Name CD19
Background CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
Alternate names: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
Gene ID 930
NCBI Accession NP_001171569
UniProt P15391
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Study of CD19 expressing Lymphocytes. Flow Cytometry: 2 g/test (5x10e5 cell/test) or 100 l blood sample. Immunohistochemistry: Working dilution 1: 25-1: 50. This antibody is suitable on cryostat sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS with 1 mg/ml BSA and no preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Expiry Date 12 months
Background publications Freedman, Nadler: "Cell surface markers in hematologic malignancies." in: Seminars in oncology, Vol. 14, Issue 2, pp. 193-212, 1987 (PubMed).

Campana, Janossy: "Leukemia diagnosis and testing of complement-fixing antibodies for bone marrow purging in acute lymphoid leukemia." in: Blood, Vol. 68, Issue 6, pp. 1264-71, 1987 (PubMed).

Nadler, Anderson, Marti et al.: "B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 244-50, 1983 (PubMed).

Melsom, Funderud, Lie et al.: "The great majority of childhood lymphoblastic leukaemias are identified by monoclonal antibodies as neoplasias of the B-cell progenitor compartment." in: Scandinavian journal of haematology, Vol. 33, Issue 1, pp. 27-34, 1984 (PubMed).

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