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CD19 antibody (AA 485-556)

Name: CD19 antibody (AA 485-556)
SKU: ABIN724595
Price: 384.62 USD
Availability: InStock

CD19 Reactivity: Human, Mouse, Rat, Sheep FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Human Raji cells probed with CD19 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-CD19 Polyclonal Antibody (ABIN724595), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Rabbit Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-CD19 Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

5 images

(3 references)

Catalog No. ABIN724595

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
28

19

16

16

10

7

5

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 485-556
Reactivity
292

161

47

30

18

10

7

6

3

3

2

2

2

2

2

2

2

2
Human, Mouse, Rat, Sheep
Host
172

139

47

4
Rabbit
Compatible Secondaries
Clonality
231

127

2
Polyclonal
Conjugate
111

22

21

19

16

8

8

8

7

7

7

7

7

7

7

6

6

6

4

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
262

116

71

62

60

39

22

19

17

13

13

9

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Mouse, Rat, Sheep

Predicted Reactivity

Cow,Pig,Horse,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from rat CD19

Isotype

IgG
anti-CD19 Molecule (CD19) (AA 151-250) antibody
CD19 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 Reactivity: Human FACS, WB, IF, IHC, ELISA Host: Mouse Monoclonal 8G11B7 unconjugated

anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 Reactivity: Human, Mouse, Monkey, Rat, Sheep, Pig, Cow, Guinea Pig, Dog, Avian, Cat, Chicken, Donkey, Goat, Hamster, Horse, Rabbit WB Host: Goat Polyclonal unconjugated

anti-CD19 Molecule (CD19) (pTyr531) antibody
CD19 Reactivity: Human, Mouse FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 Reactivity: Human FACS, IHC, ELISA, Coat, StM Host: Mouse Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 Reactivity: Human FACS, WB, IF, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 Reactivity: Human WB, IP, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, ICC Host: Mouse Monoclonal 4G7 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, IP Host: Mouse Monoclonal LT19 unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Takahashi, Ikezumi, Saitoh: "Rituximab protects podocytes and exerts anti-proteinuric effects in rat adriamycin-induced nephropathy independent of B-lymphocytes." in: Nephrology (Carlton, Vic.), Vol. 22, Issue 1, pp. 49-57, (2016) (PubMed).

Reese, Wilson, Huang, Redfield, Zhong, Djamali: "Calcineurin Inhibitor Minimization With Ixazomib, an Investigational Proteasome Inhibitor, for the Prevention of Antibody Mediated Rejection in a Preclinical Model." in: Transplantation, (2015) (PubMed).

Goodwin, Choi, Hsieh, Neugent, Ahn, Hayenga, Singh, Shackelford, Lee, Shulaev, Dhar, Takeda, Kim: "Targeting Hypoxia-Inducible Factor-1α/Pyruvate Dehydrogenase Kinase 1 Axis by Dichloroacetate Suppresses Bleomycin-induced Pulmonary Fibrosis." in: American journal of respiratory cell and molecular biology, Vol. 58, Issue 2, pp. 216-231 (PubMed).
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Synonyms

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background

Synonyms: AW495831, B-lymphocyte antigen CD19, Differentiation antigen CD19, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Gene ID

12478

UniProt

F1LNH2

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway