Desmoplakin antibody

Catalog No. ABIN111818

Product Details anti-Desmoplakin Antibody

Target: Desmoplakin (DSP)

Reactivity: Human, Mouse, Rat, Chicken, Cow, Amphibian, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, Rat MHC1C1 cell line, Bovine cells: MDBK, BMGE.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse and Chicken.

Purification: Affinity Chromatography on Protein A

Immunogen: Bovine Desmoplakin 1 and 2

Clone: DP2-15

Isotype: IgG1

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water.

Buffer: Final solution contains PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: Desmoplakin (DSP Products)

Background: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

Gene ID: 1832

NCBI Accession: NP_001008844

UniProt: P15924

Pathways: Cell-Cell Junction Organization