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Desmoplakin antibody

DSP Reactivity: Human, Mouse, Rat, Chicken, Cow, Amphibian, Dog WB, IHC (fro), IF Host: Mouse Monoclonal DP2-15 unconjugated
Catalog No. ABIN111818
  • Target See all Desmoplakin (DSP) Antibodies
    Desmoplakin (DSP)
    Reactivity
    • 32
    • 14
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Cow, Amphibian, Dog
    Host
    • 28
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 31
    • 2
    Monoclonal
    Conjugate
    • 22
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Desmoplakin antibody is un-conjugated
    Application
    • 14
    • 11
    • 10
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
    Specificity
    This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, Rat MHC1C1 cell line, Bovine cells: MDBK, BMGE.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Bovine, Rat, Mouse and Chicken.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Bovine Desmoplakin 1 and 2
    Clone
    DP2-15
    Isotype
    IgG1
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    Discover our top product DSP Primary Antibody
  • Application Notes
    Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1 mL dist. water.
    Buffer
    Final solution contains PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.
  • Target
    Desmoplakin (DSP)
    Alternative Name
    Desmoplakin (DSP Products)
    Background
    Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP
    Gene ID
    1832
    NCBI Accession
    NP_001008844
    UniProt
    P15924
    Pathways
    Cell-Cell Junction Organization
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