LY75/DEC-205 antibody

Catalog No. ABIN111868

Product Details anti-LY75/DEC-205 Antibody

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LY75/DEC-205 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells. Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205.

Purification: Affinity Chromatography.

Immunogen: Mouse lymph node tissue.

Clone: NLDC145

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/400). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.02 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: Do Not Use Sodium Azide as preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: CD205 / DEC-205 (LY75 Products)

Background: Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.Synonyms: C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6

Gene ID: 17076

NCBI Accession: NP_038853

UniProt: Q60767