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Independently validated Antibodies

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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245 results:

RFP antibody

RFP Reactivity: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

356 references

Independent Validation

1 validation

Catalog No. ABIN129578

$350.00

Plus shipping costs $50.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

Datasheet as PDF

Collagen Type I antibody

COL1 Reactivity: Human, Rat, Mouse, Cow, Mammalian ELISA, WB, IHC, IP Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Oridonin suppresses α-SMA and ECM protein expression.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

46 images

62 references

Independent Validation

2 validations

Catalog No. ABIN5596819

$548.90

Plus shipping costs $50.00

100 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

Datasheet as PDF

DYKDDDDK Tag antibody

WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

8 images

20 references

Independent Validation

2 validations

Catalog No. ABIN99294

$284.90

Plus shipping costs $50.00, dry ice fee $20.00 and VAT

250 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

Datasheet as PDF

TNF alpha antibody (AA 181-235)

TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IHC (fro), IF (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

Histology and immunofluorescence in rat corneal sections.

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

11 images

21 references

Independent Validation

1 validation

Catalog No. ABIN677318

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Datasheet as PDF

Caspase 8 antibody (AA 411-482)

CASP8 Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29759).

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

6 images

15 references

Independent Validation

1 validation

Catalog No. ABIN724205

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Datasheet as PDF

Recombinant SARS-CoV-2 Spike S1 antibody (RBD)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Beta, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Omicron, SARS CoV-2 Delta ELISA, IF, Crys, SPR, mIHC Host: Human Monoclonal CR3022 unconjugated Recombinant Antibody

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Mutations (indicated in orange) in the SARS-CoV-2 S Protein Receptor Binding Domain (RBD) in variants of concern (grey) and variants of note (mint).

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Biolayer interferometry was used to determine dissociation constants of SARS-BLOCK™ peptides associated with IgG neutralizing antibody (ABIN6952546).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (Trimer) protein (ABIN6952670).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (P.

Surface expression and recognition of minispike by COVID-19 patient serum.

VeroE6 cells infected over night with VSVDeltaG-minispike-eGFP (green) are recognized by IgG of S ELISA-positive patients in contrast to a control replicon expressing blue fluorescent protein (VSVDeltaG-BFP).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

17 images

8 references

Independent Validation

1 validation

Catalog No. ABIN6952546

$522.00

Plus shipping costs $50.00

200 μg

Shipping to: United States

Delivery in 7 to 11 Business Days

Datasheet as PDF

Iba1 antibody (C-Term)

AIF1 Reactivity: Human WB, IHC, IF, ICC, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032)

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

Independent Validation

1 validation

Catalog No. ABIN2857032

$609.18

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 4 Business Days

Datasheet as PDF

CTNNB1 antibody (N-Term)

KO Validated CTNNB1 Reactivity: Human WB, IHC, IF, IHC (p), FACS, IP, ICC, IHC (fro), ChIP, IHC (wm) Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

16 images

8 references

Independent Validation

1 validation

Catalog No. ABIN2855042

$609.18

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 4 Business Days

Datasheet as PDF

Dendra 2 antibody

Reactivity: Coral WB, FACS, ICC Host: Rabbit Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

5 images

16 references

Independent Validation

1 validation

Catalog No. ABIN361314

$417.45

Plus shipping costs $50.00, dry ice fee $20.00 and VAT

100 μg

Shipping to: United States

Delivery in 5 to 7 Business Days

Datasheet as PDF

MAP2K5 antibody (AA 251-350)

MAP2K5 Reactivity: Human, Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

2 references

Independent Validation

1 validation

Catalog No. ABIN754183

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Datasheet as PDF

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