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Independently validated Antibodies

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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212 results:

anti-RFP antibody (Red Fluorescent Protein) Primary Antibody

RFP Reactivity: Discosoma ELISA, FACS, IF, IHC, IHC (fro), IHC (p), IP, WB Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

353 references

1 validation

Catalog No. ABIN129578

$295.00

Free shipping!

100 μL (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

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anti-Collagen Type I antibody (Collagen, Type I) Primary Antibody

Antigen: collagen type III alpha 1 chain COL1 Reactivity: Human, Mouse, Rat, Cow, Mammalian ELISA, IHC, IP, WB Host: Rabbit Polyclonal unconjugated

Immunofluorescence staining of extracellular and intracellular type I collagen.

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

42 images

62 references

1 validation

Catalog No. ABIN5596819

$670.50

Plus shipping costs $45.00

100 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

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anti-DYKDDDDK Tag antibody Primary Antibody

IP, ELISA, WB Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

8 images

20 references

2 validations

Catalog No. ABIN99294

$358.50

Plus shipping costs $45.00 and $22.00 dry ice

250 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

Details

anti-Iba1 antibody (Allograft Inflammatory Factor 1) C-Term Primary Antibody

AIF1 Reactivity: Human, Mouse, Rat IHC (fro), IHC (p), IHC, WB Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

1 validation

Catalog No. ABIN2857032

$509.08

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 3 to 4 Business Days

Details

anti-Caspase 8 antibody (CASP8) AA 411-482 Primary Antibody

CASP8 Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29759).

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

6 images

15 references

1 validation

Catalog No. ABIN724205

$374.00

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Details

anti-TNF alpha antibody (Tumor Necrosis Factor alpha) AA 181-235 Primary Antibody

TNF alpha Reactivity: Human, Mouse, Rat, Horse, Rabbit ELISA, IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Histology and immunofluorescence in rat corneal sections.

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 images

21 references

1 validation

Catalog No. ABIN677318

$374.00

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Details

anti-beta Catenin antibody (Catenin, beta) N-Term Primary Antibody

Synonym: catenin beta 1 CATNB Reactivity: Human, Mouse, Rat ChIP, CUT&RUN, ICC, IF, IHC (p), IP, WB Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin, beta (CATNB) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

6 references

1 validation

Catalog No. ABIN2855042

$509.08

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 3 to 4 Business Days

Details

anti-beta Amyloid antibody (Amyloid beta) N-Term Primary Antibody

Abeta Reactivity: Human, Mouse IHC, ELISA, WB Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide.

Western Blot of Rabbit anti-Beta Amyloid antibody.

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Image no. 7 for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

9 images

1 reference

2 validations

Catalog No. ABIN95037

$657.00

Plus shipping costs $45.00 and $22.00 dry ice

100 μg (More quantities available)

Shipping to: United States

Delivery in 1 to 2 Business Days

Details

anti-c-FOS antibody (c-Fos) N-Term Primary Antibody

c-Fos Reactivity: Human, Mouse CUT&RUN, IF, IF/ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-c-Fos (c-Fos) (N-Term) antibody (ABIN2682008)

Immunochemical staining of human FOS in human skin with rabbit polyclonal antibody (0.

Immunofluorescence staining of FOS in HeLa cells.

Immunochemical staining of human FOS in human breast carcinoma with rabbit polyclonal antibody (0.

Immunochemical staining of human FOS in human brain with rabbit polyclonal antibody (0.

6 images

2 references

1 validation

Catalog No. ABIN2682008

$259.09

Plus shipping costs $45.00

100 μL (More quantities available)

Shipping to: United States

Delivery in 11 to 13 Business Days

Details

anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) AA 31-130 Primary Antibody

RAF1 Reactivity: Human ELISA, IF (cc), IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (ABIN733208) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf/Raf1 Polyclonal Antibody (ABIN733208) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

6 images

1 reference

1 validation

Catalog No. ABIN733208

$374.00

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Details

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