S100A8 antibody

Catalog No. ABIN111890

Product Details anti-S100A8 Antibody

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This Monoclonal 8-5C2 antibody reacts with Human MRP8 in stimulated monocytes and macrophages in late phase or chronic inflammation. The antigen is MRP8, the epitope is suspected in the central portion of the peptide. Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes but not in other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stain positive. Antigen Distribution on Tissue Sections: MRP8 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP8 increasingly during the late stages of inflammation. A low MRP8 (and high MRP- 14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis or chronic rejection after allograft transplantation, MRP8 and MRP14 positive cells consist of different subpopulations.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Human. The antibody reacts with Bovine spleen and shows a diffuse reaction with Swine spleen.

Purification: Affinity Chromatography

Immunogen: Cultured human monocytes.

Clone: 8-5C2

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections: 5-10 μg/mL (1/20-1/50) (No pretreatment forantigen retrieval necessary). Microwave treatment in 0.01M Citrate, pH 6.0, may enhance the reactivity. Suggested Positive Control: Human tonsil. Has been described to work in Dot Blots and not in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2, 5 mg/mL BSA, 0.05 % Kathon

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-S100A8 antibody (ABIN111890)

Wong, Zhou, Li, Tong, Zhao, Li, Yu, Koh, Beuerman: "Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication." in: Investigative ophthalmology & visual science, Vol. 52, Issue 10, pp. 7385-91, (2011) (PubMed).

Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, (2010) (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, (2007) (PubMed).

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / Calgranulin-A / MRP8 (S100A8 Products)

Background: Monoclonal antibody 8-5C2 identifies MRP8 (also named S100A8 or Calgranulin A), the Ca2+-binding light subunit of the inflammatory L-1 protein complex. MRP8 forms Ca2+ dependent dimers or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8. The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gene ID: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins